Nanobioprobe construction of acetylcholinesterase by Using of Optical Fiber

The issue of organophosphates and their usage and effects on environment has being becoming an important issue in academia. In recent years, several methods have been developed by researchers to measure paraoxon organophosphere on a global scale. In this paper, we investigate this problem and introduce a novel Nano fiber-optic bioprobe system to detect paraoxon as an enzyme inhibitor. The fiber-optic enzyme biosensors are included an acetylcholinesterase (AChE)-immobilized by cross-linking glutaraldehyde on silica optic fiber. The measurement of reducing of products 5,5'-Dithio-bis(2-nitrobenzoic acid) using paraoxon on AChE is used as a technique to sense scheme. The environment temperature and the response time to steady signal of sensor were 25 ?C and 5 min., respectively. Also, the substrate affinity was constant and Km for AChE in control was observed as optic fiber 0.43µM. Moreover, the Vmax for AChE immobilization on Optic Fiber was constant at 0.27?A/µgprotein/min. Experimental results indicated we could detect absorbance 5,5'-Dithio-bis(2-nitrobenzoic acid) at 412 nm, Paraoxn value. Moreover, we could detect paraoxon at very low-quantity sample. We could detect the detection method from 10-9 Molar using our proposed biosensor.