Isolation &Identification of Catalase Producing Bacillus spp: A Comparative Study
- Alnahda University, Faculty of Pharmacy, Sudan.
- Elrebat University, Faculty of Pharmacy, Sudan.
- Central Lab. Ministry of Higher Education & Scientific Research, Sudan.
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Catalase, an oxidoreductase enzyme, works as a detoxification system inside living cells against reactive oxygen species formed as a by-product of different metabolic reactions. This study was carried on 50 samples collected from different locations in Khartoum State for catalase production. Twenty nine out of fifty sample (58%) showed a typical characteristics of Bacillus species, fifteen out of twenty nine (51%) showed catalase positive reaction, Five out of fifteen (33%) gave highest catalase activity using hydrogen peroxide method. The strains with higest activity were identified as B. subtilis, B. pasteurii, B. coagulans, B. sphaericus and B. alvei according to chemical and morphological characteristic. The activity of the enzyme measuring spectrophotometrically according to the decrease in the absorbance of hydrogen peroxide. B. subtilis gave highest catalase activity of 135.2 ?M/ml in 48hr. incubation, which could be a promising isolate for catalase production for commercial scale.
[Babiker Mohamed Babiker Mohamed Abd Elmahamoud Ahmed and HananMoawia Ibrahim (2016); Isolation &Identification of Catalase Producing Bacillus spp: A Comparative Study Int. J. of Adv. Res. 4 (Feb). 1206-1211] (ISSN 2320-5407). www.journalijar.com
