Molecular Detection of Pseudomonas aeruginosa Isolated from Intrauterine Devices

Pseudomonas aeruginosa is a common bacterium in the environment. In humans, it is an opportunistic pathogen that is a prevalent cause of hospital-acquired infections. Currently, routine bacteriological culture on selective/non-selective culture media is the cornerstone of microbiological detection. The aim of this study was to compare isolation rates of P. aeruginosa by conventional culture and molecular (PCR) detection of P. aeruginosa isolated from intrauterine devices. Out of the 98 intrauterine devices collected from women in three hospitals in governorate Al-Najaf, Iraq, only 20 (30.5%) isolates belonged to P. aeruginosa, identified by diagnostic tests including cultural characters, biochemical tests and confirmed by PCR technique. The PCR method of gene algD GDP mannose was the best methods for diagnosis, which has led to isolate and diagnosis 20 isolates of P. aeruginosa from intrauterine device.