ANTI PROLIFERATIVE AND APOPTOTIC EFFECTS OF PUNICA GRANTUM LAND ZIZIPHUS MAURITIANA AGAINST CARCINOMA CELLS: INVITRO AND INVIVO.

This study was designed to investigate the anticancer effects of Punica grantum Land Ziziphus mauritiana against carcinoma cells invitro and invivo. Invitro antioxidant activity of ethanol, aqueous and chloroform seed extracts of Punica grantum L and Ziziphus mauritiana showed that the ethanol extract of combination of both plants having significant Super oxide, Nitric oxide and ABTS scavenging activity with IC50 value of 32.79,31.95,78.85 ?g/ml respectively. The MTT assay indicated that the percentage decrease of HeLa cells survival was found to be 18.68% in 100 ?g of ethanol extract of combination of Punica grantum L and Ziziphus mauritiana and it also stimulated caspase-3 protease by 17.9-fold increase in DEVD-pNA cleavage.Invivo antioxidant displayed theethanol and aqueous extract of combination of both plants and ethanol extract of Punica grantum L has produced the significant increase in the super oxide dismutase and decrease in lipid peroxidation (c<0.001).The ethanol extracts of combination of both plants induced chromosomal aberrations such as spindle disturbance, bridge chromosome, sticky chromosomes, and polar deviations in root tips of onions and also showed root growth inhibition activity at part with colchicine as standard and these differences were statistically nonsignificant after 72h.Consequently, the synergic anticancer activity of ethanol extract of Punica grantum L and Ziziphus mauritiana was proved.