A NEW SPERM PREPARATION TECHNIQUE BY COMBINATION OF DENSITY GRADIENT CENTRIFUGATION AND GLASS WOOL FILTRATION TECHNIQUES VERSUS EACH ONE ALONE FOR INFERTILE MEN WITH ASTHENOZOOSPERMIA

Background:- A variety of techniques have been developed to separate motile and morphologically normal spermatozoa from other constituents of the ejaculate to optimize successful assisted reproductive techniques. Objectives:- To compare the asthenozoospermic semen outcomes of three in vitro sperm activation (ISA) techniques and evaluate the efficacy, namely; Density gradient centrifugation (DGC) technique, Glass wool filtration technique and Max pure technique(combination of DGC and GWF techniques). Methods:- Forty three infertile men with asthenozoospermia were involved in this study. Sperm parameters assessed according to WHO (2010 and 1999). Post- activation of each sample divided into three aliquots, the first one using DGC, the second using GWF, and last one using Max pure technique. Results:- A significant increase (P<0.05) of sperm motility, progressive sperm motility and normal sperm morphology when using Max pure technique as compared to DGC and GWF techniques. Also, there was a significant increase (P<0.05) for the same parameters when using DGC technique as compared to the GWF technique. Conclusions:- Using Max pure technique for semen sample with decreased in the sperm motility were superior to that of DGC and GWF techniques.