DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROSCOPIC METHOD FOR ESTIMATION OF PROBENECID IN TABLET DOSAGE FORM.
- Department of Pharmacy, Khalsa College of Pharmacy, Amritsar.
- Department of Pharmacy, Maharishi Markandeshwar Universty, Mullana-Ambala.
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The objective of this research was to develop and validate an alternative accurate method for quantitative determination of probenecid in tablet dosage form. Probenecid is a highly lipid soluble 4-[(di-propyl amino) sulfonyl] benzoic acid having consistent uricosuric action for treatment of hyperuricaemia and gout. Probenecid was estimated using dilute sodium hydroxide at 243nm and the percentage recovery was found to be 100.2 ± 0.34. The method was tested and validated for various parameters according to the ICH guidelines. In proposed method relative standard deviation value less than 2% for the routine analysis of drug in tablet form. The limit of detection and limit of quantitation were 0.5µg/ml and 1.5µg/ml respectively. Results shows that the developed method is simple, reproducible and successfully for the estimation of probenecid in tablet dosage form without the interference of common excipients.
[Rajiv kumar, Parminder Nain and Jaspreet Kaur. (2016); DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROSCOPIC METHOD FOR ESTIMATION OF PROBENECID IN TABLET DOSAGE FORM. Int. J. of Adv. Res. 4 (May). 212-219] (ISSN 2320-5407). www.journalijar.com
