DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROSCOPIC METHOD FOR ESTIMATION OF PROBENECID IN TABLET DOSAGE FORM.

The objective of this research was to develop and validate an alternative accurate method for quantitative determination of probenecid in tablet dosage form. Probenecid is a highly lipid soluble 4-[(di-propyl amino) sulfonyl] benzoic acid having consistent uricosuric action for treatment of hyperuricaemia and gout. Probenecid was estimated using dilute sodium hydroxide at 243nm and the percentage recovery was found to be 100.2 ± 0.34. The method was tested and validated for various parameters according to the ICH guidelines. In proposed method relative standard deviation value less than 2% for the routine analysis of drug in tablet form. The limit of detection and limit of quantitation were 0.5µg/ml and 1.5µg/ml respectively. Results shows that the developed method is simple, reproducible and successfully for the estimation of probenecid in tablet dosage form without the interference of common excipients.