COMPARATIVE EVALUATION OF FLUORIDE UPTAKE BY DENTINE FROM GLASS IONOMER CEMENT FUNCTIONALIZED WITH CHLORHEXIDINE HEXAMETAPHOSPHATE NANOPARTICLES AND TYPE II GIC AT 3 DAYS– IN VITRO STUDY.

ionomer cement functionalized with ChlorhexidineHexametaphosphate nanoparticles and Type II GIC. Methodology: Nine human premolars were used on which Class I cavities measuring 5mm x 3mm x 2mm were prepared using round and straight diamond abrasive. Specimens were divided into three groups. First group of teeth specimens (n=3) were filled with GIC containing novel antimicrobial nanoparticles composed of (CHX-HMP) ChlorhexidineHexametaphosphate. Second group of teeth specimens (n=3) were filled with Type II GIC .The third group of specimens (n=3) served as control where no restorative material was filled. The samples were kept at 37 °C and 100% humidity for 3 days. Each tooth was sectioned into two half’s mesio-distally using diamond disc. Teeth sections were evaluated for fluoride uptake by dentin under SEM using EDAX image analyzer software. The results were tabulated and analyzed. Result: The amount of fluoride present in Group I (Type II GIC) was in the range of 7.64 atomic wt % and Group II (CHXHMP-GIC) was 6.71 atomic wt%.Flouride was detected till depths of 200 ?m for Type II restorative GIC (Group I) and till 700?m for CHXHMP-GIC (Group II). Conclusion: Fluoride release and consequent uptake by dentine was not significantly affected by the substitution of antimicrobial nanoparticles. The fluoride penetration and its effect on enhancing remineralization with the added antimicrobial effect of CHX is promising.