Circulating Micro-RNAs as Non-invasive Molecular Biomarkers for Early Detection Breast cancer.

In recent years, circulating miRNAs have attracted a great deal of attention as promising novel markers for various diseases. We investigated their potential to serve as minimally invasive, early detection markers in early stages breast cancer in blood plasma and non-cancerous familial high risk individuals. We profiled miRNAs extracted from the plasma of early stages breast cancer patients ,stages one and two (taken at the time-point of diagnosis) and 23 familial high risk individuals as well as 39 healthy control individuals using syber green RT-qPCR. MiRNA 21 was shown to be significantly up regulated with p-value 0.0001 ,while miR-let 7c shown to be significantly down regulated in the plasma of early stages breast cancer patients with p-value 0.0160. On the other hand, both of miR-21 and miR-148b had significant differences between early stages breast cancer patients and familial non-cancerous high risk individuals. In conclusion, the identified miRNAs might be of potential use in the development of a multi markers blood-based test to complement and improve early detection of breast cancer and to test the hypothesis that miRNAs profiles reflect the risk of breast cancer in women at high risk due to strong family history. Such multimarkers blood test might for instance provide a prescreening tool, especially for younger women, to facilitate decisions about which individuals to recommend for further diagnostic tests.