The cytotoxicity behavior of Acinetobacterbaumanii supernatant on some of mammalian cell lines in vitro

The clinical isolate of Acinetobacterbaumanii wasisolated previously amongmany bacterial genusfrom some of surgical tools and hospitals halls in Baghdadand reconfirmed during routine worksof quality control unite in central public health laboratory .There is no lysesshown around their grown colonies on blood agar and skim milk agar respectively. This isolate was appeared sensitive toImipenem, ciprofloxacin, meropenem, gentamicin, amikacin, doxycycline, norfloxacin and enorfloxacin, while resistant a gainstcloxacilin, pencillinG, ceftriaxone, colistin, oxytetracyclin, cephalothin, chloramphenicol, pipracillin, ampicillin, amoxicillin, ceftazidime and co-trimoxazole respectively, However,it was appeared moderate in its behavior against cefotaxime. The bacterial supernatant non-heated and heated (100°C for 60 min) were caused morphological changes in both animal cell lines (RD and L20B) including ((detaching, accumulation, size enlargement, rounding, shrinkage, lyses and death after 96hrs of treatment).the cytotoxicity effect is not related to protease and heamolysinsecretion.Other extracellular heat stable toxins may share in pathogenicity of A.baumanii on human cells.