Comparative studies of Partial Sequence Analysis of the Varicella zoster Virus ORF 63 Isolated from Chickenpox and Herpes zoster patients from Chennai, India

ORF 4, 21, 29,62,63,66 of VZV is the genes expressed during latency. Out of which ORF 63 is found to be expressed first and abundant gene to be expressed. It is present in two copies (ORF63/70) and codes with 278 amino acids. ORF 63 is expressed in the neurons during latency. Amplified and sequenced ORF 63 of Varicella zoster from chickenpox and herpes zoster DNA successfully. The partial sequence of both was published in NCBI. The expression of IE 63 was carried out in BL21pLys cells. Bioinformatics analysis was done to compare the partial sequence of protein and nucleotide sequence of ORF 63 of Varicella zoster and Herpes zoster with the ones in NCBI, from which it was inferred that the sequence of ORF 63 of clinical isolates, Chennai was different of a sequence of NCBI. The authors are happy to communicate the fact that this is the first work on sequencing of Varicella zoster ORF 63 and Herpes zoster ORF 63 from samples collected from vesicles sample of chickenpox and herpes zoster in Chennai, India. Thus the authors claim that different isolates of Varicella zoster and herpes zoster prevail in Chennai, India.