20Jan 2017

EFFECT OF PLANT GROWTH REGULATORS ON IMMOBILIZED CELL CULTURE OF ADHATODA VASICA NEES.

  • Institute of Professional Studies-College of Pharmacy, Gwalior, M.P. 474001. Hi-Tech College of Pharmacy, Hi-Tech Medical College and Hospital Campus, Bhubneswar, Odisha, 751025. PG Department of Pharmacology, M.K.C.G. Medical College, Brahmapur, GM, Odisha.
Crossref Cited-by Linking logo
  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

Immobilization have been suggested as a strategy to improve the overall productivity in plant tissue culture. Two auxin and one cytokine used to manipulate the media and the effect was studies in terms of cell growth. Secondary metabolite production was analyzed by HPLC. The calcium alginate concentration was optimized on the basis of cell viability and cell number increase was observed in immobilized condition.


  1. Brodelius, P.,Deus, B., Mosbach, K., and Zenk, M. H. (1979): Immobilized plant cells for the production and transformation of natural products. FEBS Letters, 103: 93 – 97
  2. Haigh, J.R., Linden, J.C. (1989): Phenolicsproductino by encapsulated Nicotianatabacum cells. Plant Cell Reports, 8: 475 - 478.
  3. Indian Herbal Pharmacopoeia, Joint publication of CSIR and Regional Research Laboratory, Jammu (India), 1992; 1-13.
  4. Johnson, T. S., Ravishankar, G. A. and Venkataraman, L. V. (1996), Biotransformation of ferulic acid and vanyllylamine to capsicin and vanillin in immobilized cell culters of Capsicum frutescens, Plant Cell, Tissue and organ Culture, 44: 117 - 121.
  5. Atal, C.K. (1980): Chemistry and Pharmacology of vasicine: A new oxytocin and abortifacient. Indian Drugs, 15: 15-18.
  6. Rao, S. R. and Ravishankar, G. A., (2000): Biotransformation of protocatechuic aldehyde and caffeic acid to vanillin and capsaicin in freely suspended and immobilized cell cultures of Capsicum frutescens. Journal of Biotechnology, 76: 137 - 146.
  7. Vasilev, N., Schmitz, C., Grömping, U., Fischer, R., Schillberg, S (2014):Assessment of Cultivation Factors that Affect Biomass and Geraniol Production in Transgenic Tobacco Cell Suspension Cultures. PLOS One, 9 (8): 1-7.
  8. Williams, P. D. and Mavituna, F., (1992). Immobilized Plant Cells, in Plant Biotechnology: Comprehensive Biotechnology, Second Supplement, Fowler, M. W., Warren, G. S. and Moo-Young, M., Pergamon Press, Oxford.
  9. Srivastava AS, Saxena HP, Singh DR. Adhatodavasica, a promising insecticide against pests of storage. Lab Dev 1965; 3(2):138.

[Chauhan Pradeep, Panigrahi Mahendra Kumar, Behera Jayanti Prava. (2017); EFFECT OF PLANT GROWTH REGULATORS ON IMMOBILIZED CELL CULTURE OF ADHATODA VASICA NEES. Int. J. of Adv. Res. 5 (1). 252-254] (ISSN 2320-5407). www.journalijar.com


Pradeep Chauhan
Institute of Professional Studies-College of Pharmacy, Gwalior, M.P. 474001

DOI:


Article DOI: 10.21474/IJAR01/2745       DOI URL: http://dx.doi.org/10.21474/IJAR01/2745


Share this article