18Feb 2017

COMPARATIVE STUDY FOR THE ASSESSMENT OF TISSUE ENGINEERED OSTEOCHONDRAL GRAFT VERSUS AUTOGENOUS GRAFT IN CRITICAL SIZE DEFECT: AN EXPERIMENTAL STUDY ON THE TEMPOROMANDIBULAR JOINT OF DOGS.

  • Lecturer, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, October University ofModern Sciences and Art, Egypt.
  • Professor, Department of Surgery, anesthesiology, and radiology, Faculty of Veterinary Medicine, Cairo University, Egypt.
  • Assistant professor, Department of oral pathology, Faculty of Oral and Dental Medicine, Cairo University, Egypt.
  • Lecturer, Department of Oral Biology, Faculty of Dentistry, October University of Modern Sciences and Art, Egypt.
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This study was performed to demonstrate the ability of mesenchymal stem cells using biphasic scaffolds, to produce osteochondral graft, assess its function, and compare it with autogenous grafting of condylar defects. Eighteen male adult mongrel dogs ranging from 12-15 kg in weight were selected for this study and were randomly divided into twoequal groups: Group A: Tissue engineering procedure was performed in 2 stages, Stage 1: Aspiration of bone marrow samples from the canine subjects was performed, Stage 2: In vitro isolation, expansion, differentiation and characterization of Mesenchyme Stem Cells was performed, followed by implantation of the tissue engineered graft. Group B: autogenous bone grafting was performed. After 16 weeks from implantation of the grafts in the created condylar defects, the dogs were sacrificed, and bone samples were prepared for histological assessment and follow up. Microscopic and histochemical analysis revealedwell-formed tissue- engineered osteochondral graft in the test group(Group A), versus very limited cartilage regeneration in the control group (Group B).Moreover, Radiographic evaluation was performed immediate post-operatively, 6 weeks, 12 weeks, and 16 weeks postoperatively. Based on our finding, we concluded that the replacement of TMJ bone and cartilage with tissue-engineered osteochondral graft was superior to autogenous bone graft at the same step.

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[Ingy Mohamed Chehata, Shereen Wagdy Arafat, Mohamed Ayad Abdelhamid, Rehab Fawzy Kassem and Samah Mohamed Kamel. (2017); COMPARATIVE STUDY FOR THE ASSESSMENT OF TISSUE ENGINEERED OSTEOCHONDRAL GRAFT VERSUS AUTOGENOUS GRAFT IN CRITICAL SIZE DEFECT: AN EXPERIMENTAL STUDY ON THE TEMPOROMANDIBULAR JOINT OF DOGS. Int. J. of Adv. Res. 5 (Feb). 809-817] (ISSN 2320-5407). www.journalijar.com

Shereen Wagdy Arafat
October University for Modern Sciences and Arts (MSA)

DOI:

Article DOI: 10.21474/IJAR01/3218      
DOI URL: http://dx.doi.org/10.21474/IJAR01/3218

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