18Feb 2017

THE EFFECT OF ALKALOIDS, SAPONINSAND THYMOQUINONE OF NIGELLA SATIVA SEEDS ON BIOFILM PRODUCTION, MOTILITY, OUTER MEMBRANE PROTEINS AND LIPOPOLYSACCHARIDE OF SOME BACTERIA.

  • Taif University, Faculty of pharmacy, Department of microbiology, Taif, Saudi Arabia.
  • Taif University, Faculty of Science, Department of Biology , Taif, Saudi Arabia.
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Alkaloids, saponinsand thymoquinone ofNigella sativaare active against both Gram-positive and Gram-negative bacteria. Subinhibitory concentrations of theseconstituentswere tested for their effect on biofilm production, motility and the expression of the proteins and the lipopolysaccharides of the outer membrane of Gram-negative bacteria. While all the three tested constituents reduced the biofilm formation byE. coli, only saponins and alkaloids reducedthe biofilm formation inK. pneumoniae and Sal. Typhimurium. Nonetheless, alkaloids, saponinsand thymoquinone, enhanced the biofilm formation in P. aeruginosa and Sh. flexneri. On the other hand,thymoquinone inhibited the motility of Escherichia coli, P. aeruginosa and Sal. Typhimurium. There were changes in the level of expression of seven and three outer membrane proteins ofE. coli and P. aeruginosa,respectively. Amongst these changes in E. coli,the amounts of OmpF apparently decreased and those ofOmpA increased. Also, three outer membrane proteins of P. aeruginosa were affected by the phytochemicals includingOprF. The expression of the latter was increased by alkaloids. Electron microscopy revealed some morphological changes inS. aureus and P. aeruginosa. It may be concluded that thymoquinone, alkaloids and saponins affect several pathogenesis mechanisms in both Gram-positive and Gram-negative bacteria.

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[Mohamed Shohayeb and Eman Halawani. (2017); THE EFFECT OF ALKALOIDS, SAPONINSAND THYMOQUINONE OF NIGELLA SATIVA SEEDS ON BIOFILM PRODUCTION, MOTILITY, OUTER MEMBRANE PROTEINS AND LIPOPOLYSACCHARIDE OF SOME BACTERIA. Int. J. of Adv. Res. 5 (Feb). 562-571] (ISSN 2320-5407). www.journalijar.com

Eman Halawani
Taif University

DOI:

Article DOI: 10.21474/IJAR01/3186      
DOI URL: http://dx.doi.org/10.21474/IJAR01/3186

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