20Mar 2017

OMEGA-3 FATTY ACID IMPROVE NON ALCOHOLIC FATTY LIVER INDUCED BY HIGH FAT DIET IN RATS.

  • Department of Biochemistry, Faculty of Pharmacy, Beni-Suef University, Beni Suef, Egypt.
  • Department of Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
  • Department of Biochemistry, Faculty of Veterinary medicine, Beni-Suef University, Beni Suef, Egypt.
Crossref Cited-by Linking logo
  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

Dietary composition can influence the development of Nonalcoholic fatty liver disease (NAFLD). The amount and type of dietary fat may directly affect liver fat content, with high-fat diets being potentially harmful. A higher dietary fat intake is associated with liver inflammation and NAFLD. An Omega-3 fatty acid used in this study as a treatment to modulate these metabolic disturbances. This study was designed to elucidate the effect of Omega-3 fatty acid on high fat diet induced NAFLD in albino Wister rats. NAFLD induced by allowing rats to feed on the high fat diet for 20 weeks. Omega-3 fatty acid was used in doses of 0.8 mg/Kg body weight. Plasma glucose , triglycerides and non-esterified fatty acids (NEFA) were measured by enzymatic method. It can be concluded that administration of high fat diet caused NAFLD in rats via abnormal or extensive fat accumulation when energy intake exceed energy expenditure. Omega-3 fatty acid succeeded in ameliorating the deleterious effects of high fat diet induced NAFLD.

  1. Schenker, M. Nonalcoholic steatohepatitis: What we know in the new millennium. The American Journal of Gastroenterology, (2002) 97(11), 2714-2724.
  2. McAvoy, N. C.; Ferguson, J. W.;? Campbell, I. W. ?and Hayes, P. C. Non-Alcoholic fatty liver disease: natural history, pathogenesis and treatment. Br J Diabetes Vasc Dis, ?2006; 6 (6) , 251-260.
  3. Donnelly KL, Smith CI, Schwarzenberg SJ, Jessurun J, Boldt MD, Parks EJ. Sources of fatty acids stored in liver and secreted via lipoproteins in patients with nonalcoholic fatty liver disease. J Clin Invest. 2005;115(5):1343-1351.
  4. Tanaka N, Sano K, Horiuchi A, Tanaka E, Kiyosawa K, Aoyama T. Highly purified eicosapentaenoic acid treatment improves nonalcoholic steatohepatitis. J Clin Gastroenterol. 2008;42(4):413-418.
  5. Kien CL.Dietary interventions for metabolic syndrome: role of modifying dietary fats. Curr Diab Rep. 2009;9(1):43-50.
  6. Wang D, Wei Y, Pagliassotti MJ. Saturated fatty acids promote endoplasmic reticulum stress and liver injury in rats with hepatic steatosis. Endocrinology.2006;147(2):943-951.
  7. Van den Berg SA, Guigas B, Bijland S, et al. High levels of dietary stearate promote adiposity and deteriorate hepatic insulin sensitivity. Nutr Metab (Lond). 2010;7:24.
  8. Parker HM, Johnson NA, Burdon CA, Cohn JS, O'Connor HT, George J. Omega-3 supplementation and non-alcoholic fatty liver disease: a systematic review and meta-analysis. J Hepatol. 2012;56:944?51.
  9. Levy JR, Clore JN, Stevens W. Dietary n-3 polyunsaturated fatty acids decrease hepatic triglycerides in Fischer 344 rats. Hepatology 2004; 39: 608-616 [PMID: 14999679 DOI: 10.1002/hep.20093].
  10. Sato, A., et al., Antiobesity Effect of Eicosapentaenoic Acid in High-Fat/High-Sucrose Diet?Induced Obesity Importance of Hepatic Lipogenesis. Diabetes, 2010. 59(10): p. 2495-2504.
  11. Marsman, H.A., et al., Reversal of hepatic steatosis by omega-3 fatty acids measured non-invasively by (1) H-magnetic resonance spectroscopy in a rat model. J Gastroenterol Hepatol, 2011. 26(2): p. 356-63.
  12. Marsman, H.A., et al., Omega-3 fatty acids reduce hepatic steatosis and consequently attenuate ischemia-reperfusion injury following partial hepatectomy in rats. Dig Liver Dis, 2011. 43(12): p. 984-90.
  13. Duncombe, W .The colorimeteric micro-determination of non-esterified fatty acids in plasma .clinical chimica acta 1964; 9(2):122-125.
  14. Masterton GS, Plevris JN, Hayes PC. Review article: omega-3 fatty acids - a promising novel therapy for non-alcoholic fatty liver disease. Aliment Pharmacol Ther 2010; 31: 679-692 [PMID: 20415840 DOI: 10.1111/j.1365-2036.2009.04230.x]
  15. Ruderman, N.B.,D. Carling, M. Pretki and M. Cacicedo. "AMPK , insulin resistance , and the metaboilic syndrome ". the journal of clinical investigation? 2013;123 (7) :2764.
  16. Bargut, T. C. L., C. A. Mandarim- de-Lacerda and M.B. Aguila. " A high fish oil diet prevents adiposity and modulates white adipose tissue inflammation pathways in mice " the journal of international biochemistery 2015;52(6):499-513.
  17. Mollace, V., M. Gliozzi, C Carresi, V. Musolino and F. Oppedisano . Re-assessing the mechanism of action of n-3 fatty acids proceedings of the Nutrition Society 2013;69 (03):324-332.
  18. Nguyen MT,?Satoh H,?Favelyukis S,?Babendure JL,?Imamura T,?Sbodio JI,?Zalevsky J,?Dahiyat BI,?Chi NW,?Olefsky JM. JNK and tumor necrosis factor-alpha mediate free fatty acid-induced insulin resistance in 3T3-L1 adipocytes. Journal of biological chemistery? 2005;280 (42):36361-35371.
  1. 19.Nicola M., Di Minno D., Russolillo A., Lupoli R., Ambrosino P., Di Minno D. Omega-3 fatty acids for the treatment of non-alcoholic fatty liver disease. World J Gastroenterol 2012 November 7; 18(41): 5839-5847.
  2. Bugianesi E, McCullough AJ, Marchesini G. Insulin resistance: a metabolic pathway to chronic liver disease. Hepatology 2005;42:987?1000.
  3. Andre?s-Blasco.I, , Herrero-Cervera1A, Vinue.A., Herva?, PiquerasS.L., Sanz. M.,Deborah Jane Burks D.J., and Gonza?lez-Navarro. H., Hepatic lipase deficiency produces glucose intolerance, inflammation and hepatic steatosis. Journal of Endocrinology 2015; 227, 179?191.
  4. Cipolla-Neto, J., Amaral, F.G., Afeche, S.C., Tan, D.X., Reiter, R.J.,. Melatonin,energy metabolism, and obesity: a review. Pineal Res. 2014;56, 371e381.
  5. Annamalai S., Mohanam L. , Prabhu AV. Effect of combination therapy of melatonin and orlistat on high fat diet induced changes in lipid profiles and liver function parameters in serum of rats.Obesity Medicine 2 (2016) 41-45.
  6. Mantena S, Vaughn D, Andringa K, et al. High fat diet induces dysregulation of hepatic oxygen gradients and mitochondrial function in vivo. Biochem J 2009;417:183?93.
  7. Green CJ, Hodson L. The influence of dietary fat on liver fat accumulation. Nutrients 2014;6:5018?33.
  8. Shearer GC, Savinova OV, Harris WS.. Fish oil?how does it reduce plasma triglycerides? Biophys. Acta .2012;1821:843?51 M
  9. Horakova O?, Medrikova D?, van Schothorst EM, Bunschoten A, Flachs P, Kus V,Kuda O, Bardova K,Janovska K, Hensler M,Rossmeisl M, Wang-Sattler R,Prehn C, Kopeckk J.?Preservation of Metabolic Flexibility in Skeletal Muscle by a Combined Use ofn-3 PUFA and Rosiglitazone in Dietary Obese Mice. PLoS ONE 2012; 7(8):e43764.
  10. Tanaka Zhang, E. Sugiyama, H. Kono, A. Horiuchi, T. Nakajima, H. Kanbe, E.Tanaka, F.J. Gonzalez, T. Aoyama, Eicosapentaenoic acid improves hepatic steatosis independent of PPARalpha activation through inhibition of SREBP-1 maturation in mice, Biochem. Pharmacol. 80 (2010) 1601?1612

[Reem M. Hashem, Laila A. Rashed, Ghada M. Safwat and Ibrahim T. Ibrahim. (2017); OMEGA-3 FATTY ACID IMPROVE NON ALCOHOLIC FATTY LIVER INDUCED BY HIGH FAT DIET IN RATS. Int. J. of Adv. Res. 5 (Mar). 505-512] (ISSN 2320-5407). www.journalijar.com

ibrahim taha inrahim
Department of Biochemistry, Faculty of Pharmacy, Beni Suef University, Beni Suef, Egypt

DOI:

Article DOI: 10.21474/IJAR01/3539      
DOI URL: http://dx.doi.org/10.21474/IJAR01/3539

Download Full Paper

Download PDF No. of Downloads: 20 | No. of Views: 72