Biochemical and Histopathological study of aqueous and methanolic extracts of Datura innoxia on Wistar rats

Different parts of Datura innoxia have been identified traditionally to possess various medicinal uses in Sudan and Africa such as skin treatment eruptions, colds, nervous disorders, narcotic for surgical procedures, anti-spasmodic, anti-asthmatic, narcotic, anti-microbial agent and neuro-sedative. This study was done for evaluate the toxicity of methanolic and aqueous extraction of seeds and leaves of the Datura innoxia on Wistar rats by using two different doses in three weeks orally consumption. Rats were allotted at random to nine groups, each group contains 6 rats; group one served as control, group two and three treated with 100 and 300 mg kg-1 of methanol extraction of seeds, group four and five treated with 100 and 300 mg kg-1 of methanol extracted of leaves, group six and seven treated with 100 and 300 mg kg-1 of water extracted of seeds, group eight and nine treated with 100 and 300 mg kg-1 water extract of leaves. The results revealed significant decrease (p ? 0.05) in body weight gain for rats in groups 3, 7 and 8 after the three weeks, while in the other groups there is no significant change in the body weight gain comparing to the control group and also it is clearly observed that an alteration in enzyme of aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP) activities. Changes in concentration of cholesterol, urea, serobiochemical parameters and pathological changes in vital organs were also observed. The results concluded that Datura innoxia is toxic causing due to alteration in various serobiochemical and hematological parameters, this toxicity correlated with dysfunction of vital organs (kidney, liver and brain) due to present of toxic material in plant studied.