17Jul 2017

IN VITRO ANTIOXIDANT ACTIVITIES OF ETHANOLIC EXTRACT AND TRITERPENOID FRACTION OF GARCINIA KOLA STEM BARK AND INHIBITION OF ARSENIC-INDUCED RAT LIVER AND KIDNEY MITOCHONDRIAL MEMBRANE PERMEABILITY TRANSITIONS.

  • Department of Biochemistry, Benjamin S. Carson (Snr.) School of Medicine, College of Health and Medical Sciences, Babcock University, Ilisan-Remo, Ogun State, Nigeria.
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This study was designed to investigate the in vitro antioxidant activities and inhibitory effects of G. kola stem bark ethanolic extract (EEGK) and triterpenoid fraction (TFGK) on arsenic-induced liver and kidney mitochondrial membrane permeability transitions (MMPTs). DPPH (1-1-diphenyl 2-picryl hydrazyl), hydrogen peroxide scavenging (HPSA), hydroxyl radical scavenging (HRSA) and total antioxidant capacity (TAC) assays were performed to determine antioxidant activity in vitro. Rat liver and kidney mitochondrial fractions were isolated using differential centrifugation technique while MMPT assay was performed using spectrophotometric method. Results showed that EEGK had a significantly (P<0.05) high DPPH, HPSA, HRSA and TAC scavenging activity than TFGK. However, 30 μg/mL TFGK significantly (P<0.05) inhibited arsenic-induced liver and kidney MMPT by 92.59% and 88.89% when compared with EEGK (70.37% and 66.67%) and standard spermine (81.48% and 77.78%). Thus, data from this study indicated that ethanolic extract of G. kola stem bark exhibited higher antioxidant activity in vitro while TFGK substantially inhibited arsenic-induced liver and kidney mitochondrial membrane permeability transitions.

  1. Adesina, S. K., Z. O. Gbile, O. A. Odukoya, D. D. Akinwusi, H. C. Illoh, and A. A. Yeola. 1995. Survey of indigenous useful plants of West Africa with special emphasis on medicinal plants and issues associated with management, The United Nations Programme on Natural Resources in Africa.
  2. Anyasor, G. N., F. D. Onajobi, O. Osilesi, and O. Adebawo. 2014. Hexane fraction of Costus afer ker Gawl leaves inhibited mitochondrial membrane permeability transition, F1F0 Adenosine triphosphatase and scavenged nitric oxide and hydrogen peroxide, J. Investigational Biochem. 3:78-84.
  3. Araujo, M., B. Filipa, R. C. Alves, and S. Pimentel. 2015. Phenolic compounds from olive mill wastes: health effects, analytical approach and application as food antioxidants. Trends Food Sci. Technol. 45:200-211.
  4. Bishayi, S. 2000. Sodium arsenite induced alteration in functional activity of murine peritoneal macrophages. Indian J. Pharmacol. 32:192-197.
  5. Buhian, W. P. 2016. Bioactive metabolite profiles and antimicrobial activity of ethanolic extracts from Muntingia calabura L. leaves and stems. Asian Pac. J. Trop. Biomed. 6:682-685.
  6. Bustamante, J., L. Nutt, S. Orrenius, and V. Gogvadze. 2005. Arsenic stimulates release of cytochrome c from isolated mitochondria via induction of mitochondrial permeability transition, Toxicol. Appl. Pharmacol. 207:110-116.
  7. Chandranayagam, C., G. Veeraraghavan, A. Subash, and H. R. Vasanthi. 2013. Restoration of arsenite induced hepato-toxicity by crude tannin rich fraction of Theobroma cacao in Sprague Dawley rats. Food Res. Int. 50:46-54.
  8. Chavan, H., P. Christudoss, K. Mickey, R. Tessman, H. Ni, R. Swerdlow, and P. Krishnamurthy. 2017. Arsenite effects on mitochondrial bioenergetics in human and mouse primary hepatocytes follow a nonlinear dose response. Oxid. Med. Cell. Longev. https://doi.org/10.1155/2017/9251303.
  9. Farombi, E. O., and O. Owoeye. 2011. Antioxidative and chemopreventive properties of Vernonia amygdalina and Garcinia biflavonoid. Int. J. Environ. Res. Public Health 8:2533-2555.
  10. Flora, S. J., A. Mehta, and R. Gupta. 2009. Prevention of arsenic-induced hepatic apoptosis by concomittant administration of garlic extracts in mice. Chem. Biol. Interact. 177:227-233.
  11. Halliwell, B., J. M. Guttridge, and O. I. Aruoma. 1987. The deoxyribose method: a simple "test-tube" assay for determination of rate constants for reactions of hydroxyl radicals. Anal. Biochem. 165: 215-219.
  12. Hogeboom, G. H., W. C. Schneider, and G. E. Pallade. 1948. Cytochemical studies of mammalian tissues; isolation of intact mitochondria from rat liver; some biochemical properties of mitochondria and submicroscopic particulate material. J. Biol. Chem. 172:619-635.
  13. Hosseini, M. J., F. Shaki, M. Ghazi-Khansari, and J. Pourahmad. 2013. Toxicity of arsenic (III) on isolated liver mitochondria: A new mechanistic approach. Iranian J. Pharma Res. 12:121-138.
  14. Iwu, M. M.1993. Handbook of African Medicinal Plants. Boca Raton, Florida: CRC Pres,
  15. Juliana, A., S. Moctar, and K. Christopher. 2006. Garcinia kola Heckel, Forest and Landscape 2:264-269.
  16. Iwu, M. M., O. A. Igboko, C. O. Okunji, and M. S. Tempesta. 2009. Antidiabetic and aldose reductase activities of Biflavanones of Garcinia kola. J. Pharm. Pharmacol. 42:290-292.
  17. Jomova, K., Z. Jenisova, M. Feszterova, S. Baros, J. Liska, D. Hudecova, C. J. Rhodes, and M. Valko. 2011. Arsenic: toxicity, oxidative stress and human disease. J. Appl. Toxicol. 31:95-107.
  18. Kapaj, S., H. Peterson, K. Liber, and P. Bhattacharya. 2006. Human health effects from chronic arsenic poisoning-a review. J. Environ. Sci. Health, Part A 41:2399-2428.
  19. Katerina, K., and C. Gonzola. 2016. Singlet oxygen: applications in biosciences and nanosciences. Royal Soc. Chem. 1:12-25.
  20. Kitchin, K. T. 2001. Recent advances in arsenic carcinogenesis: modes of action, animal model systems, and methylated arsenic metabolites. Toxicol. Appl. Pharmacol. 172:249-261.
  21. Kulp, R. T., H. E. Shelley, and O. S. Ronald. 2004. Redox transformations of arsenic oxyanions in periphyton communities. Appl. Environ. Microbiol. 70:6428-6434.
  22. Lapidus, R. G., and P. M. Sokolove. 1993. Spermine inhibition of the permeability transition of isolated rat liver mitochondria: an investigation of mechanism. Arch. Biochem. Biophys. 306:246-253.
  23. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurment with folin phenol reagent. J. Biol. Chem. 193:265-275.
  24. Mensor, L. I., F. S. Menezes, G. G. Leitao, A. S. Reis, T. C. Santos, C. S. Coube, and S. G. Leitao. 2001. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother. Res. 15:127-130.
  25. Nasri, H., and H. Shirzad. 2013. Toxicity and safety of medicinal plants. J. HerbMed Pharmacol. 2:21-23.
  26. National Research Council. 2011. Guide for the Care and Use of Laboratory Animals (8 ed.). Washington D.C. The National Academies Press.
  27. Noha, A., I. A. Mohammed, A. Mohamed, and E. B. Elfadil. 2011. Nutritional evaluation of sorghum flour (Sorghum bicolor L. Moench) during processing of Injera. World Academy Sci. Engin. Technol. 75:86-112.
  28. Okwu, D. E., and C. Josiah. 2006. Evaluation of the chemical composition of two Nigerian medicinal plants. Afr. J. Biotechnol. 5:357-361.
  29. Onyilagba, J. C., and S. Islam. 2011. Flavonoids and other polyphenols of the cultivated species of the genus phaseolus. Int. J. Agric. Bol. 11:231-234.
  30. Ordonez, A. L., J. D. Gomez, M. A. Vattuone, and M. I. Isla. 2006. Antioxidant Activities of Sechium edule (Jacq). Food Chem. 97:452-458.
  31. Osadeba, P. O. 2012. Phytochemical analysis, hepatoprotective and antioxidant activity of Alchornea cordifolia methanol leaf extract on carbon tetrachloride-induced hepatic damage in rats. Asian Pac. J. Trop. Med. 5:289-293.
  32. Pramod, C., M. V. Neethu, V. M. Anjumol, A. Vysakh, and R. Selvaraj. 2016. Triterpenoid fraction isolated from Euphorbia tirucalli Linn. ameliorates collagen induced arthritis in Wistar rats. J. Appl. Pharma. Sci. 6:70-75. doi:10.7324/JAPS.2016.600112.
  33. Priesto, P., M. Pineda, and M. Aguilar. 1999. Spectrophotometric quantitation of antioxidant capacity through the formation of phosphomolybdenum complex: specific application to the determination of vitamin E. Anal. Biochem. 269:337-341.
  34. Rao, V. K., E. A. Carlson, and S. S. Yan. 2014. Mitochondrial permeability transition pore is a potential drug target for neurodegeneration. Biochimi. Biophys. Acta (BBA)-Mol. Basis Dis. 1842:1267-1272.
  35. Ruch, R. J., S. J. Cheng, and J. E. Klaunig. 1989. Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from chinese green tea. Carcinogenesis 10:1003-1008.
 
  1. Santra, A., A. Maiti, S. Das, S. Lahiri, S. K. Charkaborty, and D. N. Mazumder. 2000. Hepatic damage caused by chronic arsenic toxicity in experimental animals. J. Toxicol. Clin. Toxicol. 38:395-405.
  2. Schmeisser, S., Schmeisser, S. Weimer, M. Groth, S. Priebe, E. Fazius, D. Kuhlow, J.W. Einax, R. Guthke, M. Platzer, K. Zarse, and M. Ristow. 2013. Mitochondrial hormesis links low-dose arsenite exposure to lifespan extension. Aging Cell. 12:508-517.
  3. Selvaraj, V., M. Cohenford, M. Y. Armistead, and E. Murray. 2013. Arsenic trioxide (As2O3) induces apoptosis and necrosis mediated cell death through mitochondrial membrane potential damage and elevated production of reactive oxygen species in PLHC-1 fish cell line. Chemosphere 1201-1209. doi:10.1016/j.chemosphere.2012.09.039.
  4. Shalaby, E. A., and S. M. Shanab. 2013. Antioxidant compounds, assays of determination and mode of action. Afr. J. Pharm. Phamacol. 7:528-539.
  5. Sharmin, T., N. Ahmed, A. Hossain, M. M. Hossain, S. C. Mondal, M. R. Haque, M. Almas, and M. A. B. Siddik. 2016. Extraction of bioactive compound from some fruits and vegetables (pomegranate peel, carrot and tomato). Am. J. Food Nutr. 4:8-19.
  6. Shi, H., Shi, X., and K. J. Liu. 2004. Oxidative mechanism of arsenic toxicity and carcinogenesis. Mol. Cell. Biochem. 255:67-78.
  7. Singleton, V. L., R. Orthofer, and R. M. Lamuela-Ruventos. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymol. 299:152-178.
  8. Tapio, S., and B. Grosche. 2006. Arsenic in the aetiology of cancer. Mutat. Res. 612:215-246.
  9. Tentscher, P. R. 2013. On the nature of interactions of radicals with polar molecules. J. Phys. Chem.117:12560-12568.
  10. World Health Organization. 2004. Environmental Health Criteria 224: Arsenic and arsenic compounds. http://www.who.int/ipcs. Retrieved on April, 2016.

[Godswill N. Anyasor and Adesola J. Ajagunna. (2017); IN VITRO ANTIOXIDANT ACTIVITIES OF ETHANOLIC EXTRACT AND TRITERPENOID FRACTION OF GARCINIA KOLA STEM BARK AND INHIBITION OF ARSENIC-INDUCED RAT LIVER AND KIDNEY MITOCHONDRIAL MEMBRANE PERMEABILITY TRANSITIONS. Int. J. of Adv. Res. 5 (Jul). 724-733] (ISSN 2320-5407). www.journalijar.com

Godswill N. Anyasor
Department of Biochemistry, Benjamin S. Carson (Snr.) School of Medicine, College of Health and Medical Sciences, Babcock University, Ilisan-Remo, Ogun State, Nigeria.

DOI:

Article DOI: 10.21474/IJAR01/4773      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4773

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