20Jul 2017

ACUTE TOXICITY AND PATHOLOGICAL EFFECT OF ANNONA SQUAMOSA SEED AND LEAF EXTRACTS ON THE TISSUES OF CYPRINUS CARPIO.

  • Ph.D. Research Scholar, Department of Zoology, Osmania University, Hyderabad, Telangana, India.
  • Assistant Professor, Department of Zoology, Osmania University, Hyderabad, Telangana, India.
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Toxicity assessment of aqueous extracts of dried leaf and seeds of Annona squamosa to common carp ? Cyprinus carpio. It?s a plant well known for its insecticidal and pesticidal efficacy. The 96hr Lc50 value of aqueous leaf and seed extracts obtained was 300ppm and 70ppm respectively. 1/10th of the Lc50 value i.e., 30ppm and 7ppm respectively for leaf and seed extracts was taken as sub-lethal concentrations. Fishes were exposed to the respective sub-lethal concentrations for the period of 14 days. At the end of 7th day and 14th day of exposure the gill and intestine were collected from exposed and control fishes. The tissues were processed and sectioned at 4?m, stained with Haematoxylin-Eosin and finally the obtained slides were observed under light microscope at 40x magnification and photographed. The pathological changes in gills of exposed fishes include fused gill lamellae, inflammatory cells and distortions in the primary and secondary gill lamellae. The changes in intestine are occurrence of lesions, disruption of villi epithelium, infiltration of inflammatory cells through the villi and necrosis. The histological changes observed were mild in leaf extract exposed tissues but severe alterations occurred in fish tissues on exposure to Annona seed extract.

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[Syeda Hina Kauser, Shoeiba Tasneem and Rafath Yasmeen. (2017); ACUTE TOXICITY AND PATHOLOGICAL EFFECT OF ANNONA SQUAMOSA SEED AND LEAF EXTRACTS ON THE TISSUES OF CYPRINUS CARPIO. Int. J. of Adv. Res. 5 (Jul). 1094-1101] (ISSN 2320-5407). www.journalijar.com

Syeda Hina Kauser
Ph.D. Research Scholar, Department of Zoology, Osmania University, Hyderabad, Telangana, India.

DOI:

Article DOI: 10.21474/IJAR01/4820      
DOI URL: http://dx.doi.org/10.21474/IJAR01/4820

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