Detection of CD4+CD25+FOXP3+ T regulatory cells in Type 1 Diabetes Patients in Iraq by Flowcytometry analysis
Background: Regulatory T cells have an important role in the control of immune reactivity against self antigens and probably play a role in pathogenesis of type 1 diabetes (T1D). This study aimed to detect of regulatory T cells in type1 diabetes patients(T1D), and compared with healthy control subjects, and determination of GAD autoantibody. Materials and Methods: 40 children and adolescents with T1D and 10 healthy subjects of matched age and sex as controls were enrolled in this study, at the AL-Kindy Specialized Center for Diabetes and Endocrinology Diabetic Center and the Central Child Hospital, their ages between (1- 18 year), are divided into three groups, newly diagnosed , chronic patients and healthy control groups. All cases were subjected to a thorough history taking, full clinical examinations and investigations which include; glutamic acid decarboxylase (GAD) autoantibody levels by using ELISA, and flow cytometric detection of regulatory T cells. Results: GAD autoantibody level was significantly higher in patient groups ( newly diagnosed and chronic patients) compared with healthy controls. The percentages of CD4+CD25+Foxp3+ cells were significantly decreased in chronic patients of T1D than controls, while the percentages of CD4+CD25+ FOXP3+Treg were not significantly different between newly diagnosed patients and controls. Negative correlations were observed between GAD autoantibody and frequency of CD4+CD25+Foxp3. Conclusion: The percentage of regulatory T cells; CD4+CD25+Foxp3+ was decreased in patients with T1D and may have a role in its pathogenesis. Their role as a prognostic significance and their relation to various complications should be explored. 1- Minsetry of Science and Technology. 2- Collage of Science for women /Baghdad University. 3- Collage of Medicine / Baghdad University
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[Haifa M. Fahad Amna N. Jassim Wifaq M. Ali (2014); Detection of CD4+CD25+FOXP3+ T regulatory cells in Type 1 Diabetes Patients in Iraq by Flowcytometry analysis Int. J. of Adv. Res. 2 (6). 0] (ISSN 2320-5407). www.journalijar.com
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