24Sep 2017

EFFECT OF LOW LEVEL LASER ON BACTERIAL ISOLATIONS FROM THE BILE.

  • Al - Muthanna Directory of Health.
  • College of Dentistry - Al- Muthanna University Iraq ? Al - Muthanna Province.
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Objective: Using laser irradiation to attenuate or kill pathogenic bacteria isolated from the human gallbladder to prepare a vaccine consists of all the bacteria isolated from the bile to use it in immunizing the patients who experts problems in their bile system. Background: A variety of microorganisms isolated from the bile responsible for causing Cholecystitis and a large number of remedies and antibiotics used to prevent the infection or to treat it. Materials & Methods: Five microorganisms isolated from forty samples taken from patients underwent cholecystectomy operations, the bacteria isolated were (Staphylococcus aureus , Listreia monocytogenes , Escherichia coli , Shigella sonnei & Salmonella typhi) ,they were killed and attenuated using diode laser, the wavelengths used were , 820 nm respectively. Forty four rabbits underwent the vaccination program, forty rabbits of them were divided in to 5 groups with 8 rabbits each and each group was further subdivided in to two subgroups with 4 rabbits each, the first subgroup was inoculated with the live attenuated vaccine while the second one was inoculated with the killed vaccine of each of the five bacterial types, the remaining 4 were injected with the mixed ( killed and attenuated vaccine of all the bacterial types) . The humeral immune response was studied using radial immunodifussion test, to determine the immunoglobulin\\\\\\\'s concentration, for the IgM, IgG and IgA, then the animals of all the groups were injected with the challenge dose. Results: Immunoglobulin concentration rates were higher in the immunized subgroup animals compared with those of the control ones. The live attenuated vaccine induced highly immune response as compared with killed vaccine. The challenge dose was given to all the animals; the control subgroup animals died while the immunized animals remain alive and healthy. Conclusions: Live attenuated and killed vaccines inoculated intraperitonial induced high immunity against the infection, live attenuated vaccine was better than the killed one in inducing immunity responses.

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[Nabeel Khudhair Hassoon and Ihsan. F. Rostum. (2017); EFFECT OF LOW LEVEL LASER ON BACTERIAL ISOLATIONS FROM THE BILE. Int. J. of Adv. Res. 5 (Sep). 921-931] (ISSN 2320-5407). www.journalijar.com

Ihsan, F. Rostum
Prof. Dr.

DOI:

Article DOI: 10.21474/IJAR01/5405      
DOI URL: http://dx.doi.org/10.21474/IJAR01/5405

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