10Sep 2017

ESTIMATION OF GLYCATED ALBUMIN, SERUM FRUCTOSAMINE AND LIPID FRACTION IN THE PATIENTS WITH THYROID DISORDER.

  • Department of Biochemistry J.L.N. Medical College & Hospital, Ajmer, Rajasthan, 305001 INDIA.
Crossref Cited-by Linking logo
  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

Background: Thyroid disorders are the commonest disorders worldwide. Thyroid disorders leads to change in lipoprotein, carbohydrate and protein metabolism. Glycation is the process of non-enzymatic addition of sugar to protein, DNA, and lipids. Aim: To study the lipid and glycemic abnormalities in the patients of overt hypothyroidism, suclinical hypothyroidism and hyperthyroid patients, and compare the same with euthyroid subjects, Methodology: The present was conducted on 300 subjects, of either sex of various age groups. Study included diagnosed thyroid disorder patients (200) attending Medical OPD, patients admitted in medical and surgical wards and patients coming in Radioimmunoassay (RIA) laboratory, Biochemistry Department J.L.N. Medical College & Hospital, Ajmer. The results of patients were compared with the hundred healthy subjects of either sex of similar age group. The indices included:- Serum Fructosamine assayed by Nitro-Blue Tetrazolium Kinetic Method, Serum Glycated Albumin by Enzyme Linked Immunosorbent Assay, Fasting blood glucose by GOD-POD Method, serum lipid profile was estimated by enzymatic CHOD-POD for total cholesterol. Total Triglyceride by-GPO-PAP method, HDL by CHOD-POD Phosphotungtate Method, LDL cholesterol and VLDL cholesterol were calculated by Freidwald?s formula. Results: The mean serum fructosamine and glycated albumin levels were high in hypothyroid patients in comparison to healthy control, vice a versa was found in hyperthyroid patients which was statistically significant. Significant increased in level of total cholesterol, triglycerides, LDL-cholesterol VLDL cholesterol and decreased level of HDL was found in hypothyroid patients. The fasting blood glucose level were high in all the thyroid disorder patients but the results were within the reference range. Conclusion: Present study indicates that monitoring of lipid profile in patients of thyroid dysfunction would be helpful in preventing cardiovascular disease ,also serum fructosamine and glycated albumin are better short term markers of glycemic index in the patients of thyroid disorder.

  1. Abrams JJ, Grundy SM, Ginsberg H. Metabolism of plasma TG in hypothyroidism and hyperthyroidism in man. J Lipid Res.1981; 22(2): 307 22.
  2. Agdeppa D, Macaron C, Mallik T, Schnuda ND. Plasma high density lipoprotein cholesterol in thyroid disease. J ClinEndocrinolMetab. Nov.1979;49(5):726-9
  3. Allian CC, Poon LS, Chan CSG, Richmond W and Fu P: Enzymatic determination of total serum cholesterol. Clin. Chem 1974; 20: 470.
  4. Arrigo T, Wasniewska M, Crisafulli G, Lombardo F, Messina M F and Rulli I. American Diabetes Association. Executive summary: standards of medical care in Subclinical hypothyroidism: the state of the art. J Endocrinol Invest. 2008;31(1):79-84.
  5. Barker J, O?Connor J, Metcalf P. Clinical usefulness of estimation of serum fructosamine concentration as a screening test for diabetes mellitus. Br Med J.1983;287:863-70.
  6. Brennan MD, Coenen-Schimke JM, Bigelow ML, Nair SK. Changes in skeletal muscle protein metabolism and myosin heavy chain isoform messenger ribonucleic acid abundance after treatment of hyperthyroidism. J ClinEndocrinolMetab. 2006;91:4650-56
  7. Edina Billi C. Komarica, AmelaBeciragie and DzelaludinJunuzovic: The importance of HbA1C control in patients with subclinical hypothyroidism. Mater Socio Medica. October 2012; 24(2); 212-219.
  8. Finley P R, Schifman R B and Lichti D A. Cholesterol in high density lipoprotein use of mg/PTA in its enzymatic measurement. Clin. Chem. 1978; 24(6): 931-33.
  9. Fossati P and L Principe : Serum Triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clin. Chem1982 ; 28/10, 2077-2080.
  10. Friedewald W T, Levy R I and Fredrickson D S: Estimation of the concentration of low density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifugation. Clin. Chem. 1972; 18: 499-502.
  11. Hara H, Ban Y, Taniyama M, Sato R, Kushima K, Nagakura H and Kaihara M K. The significance of serum fructosamine measurement in patients with thyroid diseases. Nippon NaibunpiGakkai Zasshi.1990;66(10):1075-84.
  12. Johnson AM, Rohlfs EM, Silverman LM. Proteins. In: Burtis CA, Ash wood ER, editors. Tietz textbook of clinical chemistry. 3rd ed. Philadelphia: W.B. Saunders company; 1999
  13. Kutty M Kutty, David G .Brayant, and Nador R. Farid, Serum lipids in Hypothyroidism-A Re-evaluation. J. Clin. Endocrinol. Metabol.1978; 46 (1): 55 ? 60.
  14. Liberopoulos E N and Elisaf M S. Dyslipidemia in patients with thyroid dis orders. Hormones.2002;1(4):218-23.
  15. Mohan Kumar K M, Z Bobby, N Selvaraj, A Kumardas, B Chandra Koner and S K Sen. Possible link between glycated hemoglobin and lipid peroxidation in hyperthyroidism. ClinChem Acta.2004;342:187-92.
  16. Muls E, M Rosseneu, G Lamberigts and P Demoor, Changes in the distribution and composition of high density lipoproteins in primary hypothyroidism. Metabolism. 1985;34(4): 345 ? 353
  17. Papi G, Uberti E D, Betterle C, Carani C,E. Pearce E N and Braverman L E. Subclinical hypothyroidism. CurrOpinEndoc. Diabetes.2007;14(3):197-208.
  18. Rangaswamy R, Sreekaitha, Vivian D?souza: Fructosamine assay as a diagnostic criterion in thyroid disorder. International Journal of Medical and Health sciences. April 2013; Vol. 2; Issue 2.
  19. Regmi A, Shah B, Rai B R and Pandeya A. Serum lipid profile in patients with thyroid disorders in central nepal. Nepal M Coll J.2010;12(4): 253-256
  20. Riis A L, Jorgensen J O, Gjedde S, Norrelund H, Jurik A G and Nair K S. Whole body and forearm substrate metabolism in hyperthyroidism evidence of increased basal muscle protein breakdown. Am J PhysiolEndocrinolMetab. 2005;288:E1067-E1073
  21. Staub JJ, Althaus BU, Engler H, Ryff AS, Trabucco P, Marquardt K, Burckhardt D, Girard J, Weintraub BD. Spectrum of subclinical and overt hypothyroidism: effect on thyrotropin, prolactin, and thyroid reserve, and metabolic impact on peripheral target tissues.Am J Med. Jun 1992;92(6):631-42
  22. Sundaram V, Hanna A N, Koneru L, Newman A L and Falko J M. Both hypothyroidism & hyperthyroidism enhance low density lipoprotein oxidation. J ClinEndocrinol Metab.1997;82:3421-24. Kim H B, Han K H, Lee B W, Kim H, Lee M H and Chung E S. HbA1c and serum fructosamine levels in hyperthyroidism. J Kor. Soc.Endocriol.1992;7:46-51.
  23. Suresh BabuKondaveeti, BhanuPrakash G, I. Anand Shaker: Estimation of glycated albumin in various thyroid disorders. Int. J. Cur. Res. May 2014 Vol. 6 (09); Page 140.
  24. Surks MI, Hollo Well J.G.: Age specific distribution of severe thyrotropin and antithyroid antibodies in US population: Implication for prevalence of subclinical hypothyroidism. J. Clin. EndocrinolMetab. December 2007; 92(12): 4575-82.
  25. Trinder P: Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann. Clin. Biochem. 1969; 6: 24.
  26. Trivelli LA, Ranney HM, Lai HT : Hemoglobin components in patients with diabetes mellitus. N Engl J Med 1971; 284: 353-357.
  27. Weijers R N, Slaats E H and Kruijswijk H. Fructosamine values in hyperthyroidism, hypothyroidism and gammopathy. WeinKlin Wochenschr.1990; 180: Suppl 21-24.

[Dr. Varsha Chowdhry and Dr. G.G. Kaushik. (2017); ESTIMATION OF GLYCATED ALBUMIN, SERUM FRUCTOSAMINE AND LIPID FRACTION IN THE PATIENTS WITH THYROID DISORDER. Int. J. of Adv. Res. 5 (Sep). 230-235] (ISSN 2320-5407). www.journalijar.com

Dr Varsha Chowdhry
Department of Biochemistry J.L.N. Medical College & Hospital, Ajmer, Rajasthan, 305001 INDIA.

DOI:

Article DOI: 10.21474/IJAR01/5320      
DOI URL: http://dx.doi.org/10.21474/IJAR01/5320

Download Full Paper

Download PDF No. of Downloads: 24 | No. of Views: 87