24Sep 2017

OPTIMIZATION OF FERMENTATION CONDITIONS FOR THE ETHANOL PRODUCTION FROM SUGARCANE BAGASSE BY ZYMOMONAS MOBILIS USING RESPONSE SURFACE METHODOLOGY.

  • NUPEM, Nucleus in Ecology and Socio-environmental Development of Maca?- Federal University of Rio de Janeiro.
  • Laboratories of Bioprocess Development, Center of Technology ? School of Chemistry, Federal University of Rio de Janeiro.
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The use of biomass as renewable sources of energy has increased industrial focus toward alternative fuel because of the depletion of fossil fuel reserves, the unstable panorama of the petrol prices, the increasing environmental and political pressures. The new concept of bioethanol corresponds to its production using raw cellulosic materials, such as sugarcane bagasse. The bacterium Zymomonas mobilis was shown to be extremely attractive for the ethanol second generation production from glucose of the cellulosic fraction, due to its high capacity to absorb this sugar, resulting in high ethanol productivity values. The aim of this work was to study the influence between the medium components: Yeast Extract, KH2PO4, (NH4)2SO4, MgS04.7H20 to optimize the fermentation conditions for the ethanol production from sugarcane bagasse by Z. mobilis. Initially, to make easier the accessibility of cellulases to the cellulose microfibrils, the bagasse was submitted to a pretreatment with diluted acid to fractionate and extract the hemicellulose component from the solid residue named cellulignin and then, this solid residue was pretreated using NaOH (4%) aiming at its partial delignification. Thereafter, the pretreated cellulignin underwent the action of a commercial cellulolytic preparation, allowing the conversion from cellulose to glucose. This enzymatic pretreatment occurred under temperature of 50?C for 12 hours, after which the temperature reduced to 30?C and we inoculated the system with cells of Z. mobilis. It has been used statistical experimental design to optimize the conditions of SSF, evaluating the medium components. The optimum conditions found were 12.5 g/L of Yeast Extract, 2.5 g/L of KH2PO4; 1.5 g/L of (NH4)2SO4; 1.5 g/L of MgS04.7H20, respectively; achieving 65.3 g/L of ethanol.


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[Danielle da Silveira dos Santos Martins, Aghata Rodrigues Souza, Elcio R. Borges, Jessica D. Pe?a and Nei Pereira Jr. (2017); OPTIMIZATION OF FERMENTATION CONDITIONS FOR THE ETHANOL PRODUCTION FROM SUGARCANE BAGASSE BY ZYMOMONAS MOBILIS USING RESPONSE SURFACE METHODOLOGY. Int. J. of Adv. Res. 5 (9). 1062-1072] (ISSN 2320-5407). www.journalijar.com


Danielle Martins
Nucleus in Ecology and Socio-environmental Development of Macaé- Federal University of Rio de Janeiro

DOI:


Article DOI: 10.21474/IJAR01/5424       DOI URL: http://dx.doi.org/10.21474/IJAR01/5424


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