TRANSFER AND MOLECULAR MAPPING OF AEGILOPS TAUSCHII-DERIVED HESSIAN FLY RESISTANCE GENES (H22, H23, H24, AND H26) FROM D GENOME OF TRITICUM AESTIVUM ONTO A GENOME CHROMOSOMES OF TRITICUM TURGIDUM BY INDUCED HOMOEOLOGOUS RECOMBINATION.
- Moha Ferrahi, National Institute for Agricultural Research (INRA), Regional Center of Meknes, BP 578, Meknes, Morocco 50000.
- B. Friebe and B.S. Gill, Dept. Of Plant Pathology, Wheat Genetics Resource Center, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506-5502, USA.
- J.H. Hatchett, Dept. of Entomology and USDA-ARS, Waters Hall, Kansas State University, Manhattan, KS 66506-5502, USA.
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Aegilops tauschii Coss. (2n=14, DD) is a rich source of disease resistance genes for the improvement of cultivated wheat including several resistance genes against Hessian fly. To date, five Hessian fly resistance genes (H13, H22, H23, H24, and H26) have been transferred from Ae. tauschii to common wheat (Triticum aestivum L.). In this study, we attempted the transfer of four genes H22 (1D), H23 (6DS), H24 (3DL), and H26 (4D) from T. aestivum D genome onto A genome chromosomes of T. turgidum. The T. aestivum resistant parents WGRC01 (H22 on 1D), WGRC03 (H23 on 6DS), WGRC06 (H24 on 3DL), and WGRC26 (H26 on 4D) were crossed with T. turgidum cv. Langdon disomic substitution lines LDN 1D(1A), LDN 6D(6A), LDN 3D(3A), and LDN 4D(4A). We targeted the transfer of Hessian fly resistance genes into D-genome substitution chromosomes of T. turgidum by homologous recombination. In total 88 crosses were made. The resulting F1 plants (345 seeds) were backcrossed with the LDN 5D(5B) substitution line in which chromosome 5B is absent and replaced by a pair of 5D chromosomes with the objective of transferring D genome Hessian fly resistance genes onto A or B genomes of T. turgidum by homoeologous recombination. A total of 2,053 segregating BC1F1 plants were tested for Hessian fly resistance, and the resistant plants (1,132) were backcrossed again with LND 5D(5B) to produce BC2F1 and selfed to produce BC1F2. In the BC1F1 populations, 24 families segregated for an excess of resistant plants than the expected 1:1 resistant to susceptible plants suggesting that they were putative A-D genome positive recombinants. Mapping analysis using microsatellites was used in these families to identify recombinants between A- and D- genome chromosomes. The data indicated that H22 recombinants were recovered consisting of the distal part of the short arm of 1A, the proximal of 1DS, and the complete long arm of 1D. The recombinant can be described as T1AS-1DS1DL.
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[Moha Ferrahi, B. Friebe J.H. Hatchett and B. S. Gill. (2017); TRANSFER AND MOLECULAR MAPPING OF AEGILOPS TAUSCHII-DERIVED HESSIAN FLY RESISTANCE GENES (H22, H23, H24, AND H26) FROM D GENOME OF TRITICUM AESTIVUM ONTO A GENOME CHROMOSOMES OF TRITICUM TURGIDUM BY INDUCED HOMOEOLOGOUS RECOMBINATION. Int. J. of Adv. Res. 5 (Nov). 728-750] (ISSN 2320-5407). www.journalijar.com
National Institute for Agricultural Research (INRA), Regional Center of Meknes, BP 578, Meknes, Morocco 50000.
Article DOI: 10.21474/IJAR01/5826
DOI URL: http://dx.doi.org/10.21474/IJAR01/5826
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