15Nov 2017

TRANSFER AND MOLECULAR MAPPING OF AEGILOPS TAUSCHII-DERIVED HESSIAN FLY RESISTANCE GENES (H22, H23, H24, AND H26) FROM D GENOME OF TRITICUM AESTIVUM ONTO A GENOME CHROMOSOMES OF TRITICUM TURGIDUM BY INDUCED HOMOEOLOGOUS RECOMBINATION.

  • Moha Ferrahi, National Institute for Agricultural Research (INRA), Regional Center of Meknes, BP 578, Meknes, Morocco 50000.
  • B. Friebe and B.S. Gill, Dept. Of Plant Pathology, Wheat Genetics Resource Center, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506-5502, USA.
  • J.H. Hatchett, Dept. of Entomology and USDA-ARS, Waters Hall, Kansas State University, Manhattan, KS 66506-5502, USA.
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Aegilops tauschii Coss. (2n=14, DD) is a rich source of disease resistance genes for the improvement of cultivated wheat including several resistance genes against Hessian fly. To date, five Hessian fly resistance genes (H13, H22, H23, H24, and H26) have been transferred from Ae. tauschii to common wheat (Triticum aestivum L.). In this study, we attempted the transfer of four genes H22 (1D), H23 (6DS), H24 (3DL), and H26 (4D) from T. aestivum D genome onto A genome chromosomes of T. turgidum. The T. aestivum resistant parents WGRC01 (H22 on 1D), WGRC03 (H23 on 6DS), WGRC06 (H24 on 3DL), and WGRC26 (H26 on 4D) were crossed with T. turgidum cv. Langdon disomic substitution lines LDN 1D(1A), LDN 6D(6A), LDN 3D(3A), and LDN 4D(4A). We targeted the transfer of Hessian fly resistance genes into D-genome substitution chromosomes of T. turgidum by homologous recombination. In total 88 crosses were made. The resulting F1 plants (345 seeds) were backcrossed with the LDN 5D(5B) substitution line in which chromosome 5B is absent and replaced by a pair of 5D chromosomes with the objective of transferring D genome Hessian fly resistance genes onto A or B genomes of T. turgidum by homoeologous recombination. A total of 2,053 segregating BC1F1 plants were tested for Hessian fly resistance, and the resistant plants (1,132) were backcrossed again with LND 5D(5B) to produce BC2F1 and selfed to produce BC1F2. In the BC1F1 populations, 24 families segregated for an excess of resistant plants than the expected 1:1 resistant to susceptible plants suggesting that they were putative A-D genome positive recombinants. Mapping analysis using microsatellites was used in these families to identify recombinants between A- and D- genome chromosomes. The data indicated that H22 recombinants were recovered consisting of the distal part of the short arm of 1A, the proximal of 1DS, and the complete long arm of 1D. The recombinant can be described as T1AS-1DS1DL.


  1. Ceoloni, C., M. Biagetti, M. Ciaffi, P. Forte, and M. Pasquiri. 1996. Wheat chromosome engineering at the 4x level: the potential of different alien gene transfers into durum wheat. Euphytica 89: 87-97.
  2. Cox, T.S., and J.H. Hatchett. 1994. Hessian fly-resistance gene H26 transferred from Triticum tauschii to common wheat. Crop Sci. 34: 958-960.
  3. Cox, T.S. 1991. The contribution of introduced germplasm to the development of U.S. wheat cultivars. In: Use of plant introductions in cultivar development, Part 1 (Spec Publ no. 17). Madison, Wisconsin: Crop Science Society of America; 24-47.
  4. Dvorak, J., M.M. Noaman, S. Goyal, and J. Gorham. 1994. Enhancement of the salt tolerance of Triticum turgidum by the Kna1 locus transferred from Triticum aestivum L. chromosome 4D by homoeologous recombination. Theor Appl Genet 87: 872-877
  5. Dvorak, J., and J. Gorham. 1992. Methodology of gene transfer by homoeologous recombination into Triticum turgidum: transfer of K+/Na+ discrimination from Triticum aestivum. Genome 35: 639-646.
  6. Endo, T.R., and B.S. Gill. 1984. Somatic karyotype, heterochromatin distribution, and nature of chromosome differentiation in common wheat, Triticum aestivum em Thell. Chromosoma 89: 361-369.
  7. Friebe, B., R.G. Kynast, J.H. Hatchett, R.G. Sears, D.L. Wilson, and B.S. Gill. 1999. Transfer of wheat-rye translocation chromosomes conferring resistance to Hessian fly from bread wheat into durum wheat. Crop Sci 39: 1692-1696.
  8. Friebe, B., B.S. Gill, T.S. Cox, and F.J. Zeller. 1993. Registration of KS91WGRC14 stem rust and powdery mildew resistant T1BL?1RS durum wheat germplasm. Crop Sci 33: 220.
  9. Friebe, B., J.H. Hatchett, R.G. Sears, and B.S. Gill. 1990. Transfer of Hessian fly resistance from ?Chaupon? rye to hexaploid wheat via a T2BS?2RL wheat-rye chromosome translocation. Theor Appl Genet 79: 385-389.
  10. Friebe, B., J. Jiang, W.J. Raupp, R.A. McIntosh, and B.S. Gill. 1996. Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica 91: 59-87.
  11. Friebe, B., F.J. Zeller, and R. Kunzmann. 1987. Transfer of 1BL/1RS wheat-rye-translocation from hexaploid bread wheat to tetraploid durum wheat. Theor Appl Genet 74: 423-425.
  12. Gill, K.S., and B.S. Gill. 1994. Mapping in the realm of polyploidy: The wheat model. BioEssays vol. 16, No.11: 841-846.
  13. Gill, B.S., B. Friebe, and T.R. Endo. 1991. Standard Karyotype and nomenclature system for description of chromosome bands and structural aberrations in wheat (Triticum aestivum). Genome 34: 830-839.
  14. Gill, B.S., and W.J. Raupp. 1987. Direct genetic transfers from Aegilops squarrosa to hexaploid wheat. Crop Sci 27: 444-450.
  15. Hatchett, J.H., and R.L. Gallun. 1970. Genetics of the ability of the Hessian fly, Mayetiola destructor, to survive on wheats having different genes for resistance. Ann Entomol Soc Am 63: 1400-1407.
  16. Hatchett, J.H., and B.S. Gill. 1981. D-genome sources of resistance in Triticum tauschii to Hessian fly. J Hered 72: 126-127.
  17. Hatchett, J.H., T.J. Martin, and R.W. Livers. 1981. Expression and inheritance of resistance to Hessian fly in synthetic hexaploid wheats derived from Triticum tauschii (Coss) Schmal. Crop Sci 21: 731-734.
  18. Jauhar, P.P., O. Riera-Lizarazu, W.G. Dewey, B.S. Gill, C.F. Crane, and J.H. Bennett. 1991. Chromosome pairing relationships among the A, B, and D genomes of bread wheat. Theor Appl Genet 82: 441-449.
  19. Joppa, L.R. 1993. Chromosome engineering in tetraploid wheat. Crop Sci 33(5): 908-913.
  20. Luo, M-C, Dubcovsky, S. Goyal, and J. Dvorak. 1996b. Engineering of interstitial foreign chromosome segments containing the K+/Na+ selectivity gene Kna1 by sequential homoeologous recombination in durum wheat. Theor Appl Genet 93: 1180-1184.
  21. Ma, Z-Q, B.S. Gill, M.E. Sorrells, and S.D. Tanksley. 1993. RFLP markers linked to two Hessian fly-resistance genes in wheat (Triticum aestivum) from Triticum tauschii (Coss.) Schmal. Theor Appl Genet 85: 750-754.
  22. McIntosh, R.A., G.E. Hart, K.M. Devos, M.D. Gale, and W.J. Rogers. 1998. Catalogue of gene symbols for wheat. In A.E. Slinkard (ed.) Proc 9th Intl Wheat Genet Symp. SK. University Extension Press, University of Saskatchewan, Saskatoon, SK.
  23. Mujeeb-Kazi, A., M.D.H.M. William, and M.N. Islam-Faridi. 1996. Homozygous 1B and 1BL/1RS chromosome substitutions in Triticum aestivum and T. turgidum Cytologia 61: 147-154.
  24. Okamoto, M., E.R. Sears. 1962. Chromosomes involved in translocations obtained from haploids of common wheat. Can J Genet Cytol 4: 24-30.
  25. Pestsova, E., M.W. Ganal, and M.S. R?der. 2000. Isolation and mapping of microsatellites markers specific for the D genome of bread wheat. Genome 43: 689-697.
  26. Ratcliffe, R.H., and J.H. Hatchett. 1997. Biology and genetics of the Hessian fly and resistance in wheat. New developments in Entomology, pp: 47-55.
  27. Rao, P.M.V. 1978. The transfer of alien genes for stem rust resistance to durum wheat. P. 338-341. In Proc. Intl. Wheat Genet. Symp. 5Th, New Delhi, India.
  28. Raupp, W.J., A. Amri, J.H. Hatchett, B.S. Gill, D.L. Wilson, and T.S. Cox. 1993. Chromosomal location of Hessian fly-resistance genes H22, H23, and H24 derived from Triticum tauschii in the D genome of wheat. J Hered 84(2): 142-145.
  29. R?der, M.S., V. Korzun, K. Wendehake, J. Plaschke, M. Tixier, P. Leroy, and M. Ganal. 1998. A microsatellite map of wheat. Genetics 149: 2007-2023.
  30. Sears, E.R. 1993. Use of radiation to transfer alien segments to wheat. Crop Sci 32: 897-901.

[Moha Ferrahi, B. Friebe J.H. Hatchett and B. S. Gill. (2017); TRANSFER AND MOLECULAR MAPPING OF AEGILOPS TAUSCHII-DERIVED HESSIAN FLY RESISTANCE GENES (H22, H23, H24, AND H26) FROM D GENOME OF TRITICUM AESTIVUM ONTO A GENOME CHROMOSOMES OF TRITICUM TURGIDUM BY INDUCED HOMOEOLOGOUS RECOMBINATION. Int. J. of Adv. Res. 5 (11). 728-750] (ISSN 2320-5407). www.journalijar.com


Moha Ferrahi
National Institute for Agricultural Research (INRA), Regional Center of Meknes, BP 578, Meknes, Morocco 50000.

DOI:


Article DOI: 10.21474/IJAR01/5826       DOI URL: http://dx.doi.org/10.21474/IJAR01/5826


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