02Nov 2018

EFFECT OF PLANT GROWTH REGULATORS ON IN VITRO CALLUS INDUCTION OF SHOOT EXPLANT MANGOSTEEN (Garcinia mangostana L.)

  • Postgraduate Biology Education, Universitas Negeri Medan (UNIMED).
  • Biology Departement, Faculty of Mathematics and Natural Science, Universitas Negeri Medan (UNIMED), Indonesia.
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Introduction: The productions of generatively mangosteen still has problem because the seeds of mangosteen have recalcitrant characteristic, which means the seeds must be planted immediately, since the seeds does not have dormancy period. Aim: 1) to determine Effect of Plant Growth Regulators on in vitro Callus Induction of mangosteen (Garcinia mangostana L.), 2) to know the best combination and composition of the media to in vitro callus induction of mangosteen (Garcinia mangostana L.). Method: Researcher used complete randomized design (CRD) factorial which is consisting of 12 treatments with 3 times repetitions. The research procedures were: tool sterilization, explant sterilization, media making, explants planting. Result: The fastest response of callus appearance is on sucrose (15; 30 gr / L) and NAA (1,5; 3 ppm) treatment which is producing callus with crumb texture and color is identical to green and white. The callus has 0,53a cm in height, 1,56a cm2 in width, and 0,40a gr in weight. The percentage of callus formation is 100% and the explant response is growing all over the surface (sucrose 15 gr / L + NAA 1,5; 3 ppm). Conclusion: The plant growth regulators have effect on in vitro callus induction of shoot explant mangosteen (Garcinia mangostana L.). The best media combination and composition on in vitro callus induction of shoot explant mangosteen (Garcinia mangostana L.) were sucrose (15; 30 gr / L) and NAA (1,5; 3 ppm).


  1. Nursetiadi, E., Endang, Y., Retna, B. A. P. (2016). The influence of type of media and BAP concentration on in vitro mangosteen (Garcinia mangostana ) plant multiplication. Jurnal Bioteknologi 13 (2) : 63-72.
  2. Nugrahani, P., Sukendah dan Makziah. (2011). Propagation technique by in vitro. Basic Module 2 Of Plant Biotechnology : Jawa Timur.
  3. Sirchi, M.H.T., Muhammad, A.K., Aziz, A.A., Rashid., Arash Rafat., & Javadi, M. B. (2008). Plant regeneration as affected by plant growth regulator (PGR) in mangosteen (Garcinia mangostana). African Journal of Biotechnology 7(15), pp. 2693-2701.
  4. Harahap, F., Poerwanto, R., Suharsono, Suriani, C., & Rahayu, S. (2014). In vitro Growth and Rooting of Mangosteen (Garcinia mangostana) on Medium with Different Concentrations of Plant Growth Regulator. HAYATI Journal of Biosciences. 21(4) : 151-157.
  5. Dewi, N.M., Waeniati, & Muslimin. (2012). The influence of coconut water addition and different 2,4-D hormone concentration on MS medium to induce callus of grapes (Vitisvinifera). Jurnal Natural Science, 1 : 53-62.
  6. Winarto, B dan Rachmawati, F. (2007). Anther culture technique on Anthurium breeding. Hort. 17 (2) : 127-37.
  7. Thangene, S.R., Joshi, M.S., Khuspe S.S., dan Mascarenhas, A.E. (1994). Anther Culture in Helianthus annus, influence of genotype and culture conditions on embryo induction and plant regeneration. Plant Cell Report 13 : 222-6.
  8. Harahap, F. (2011). Tissue culture. Medan : FMIPA Unimed.
  9. Yunus, A., Samanhudi., A.T. Sakyadan M. Rahayu. (2010). Tissue culture technology. Agricultures faculty of Sebelas Maret. Surakarta.
  10. Indah, P.N, & Ermavitalini, D. (2013). Callus induction of Calophyllumino phyllum On Different Combination of 6-Benzylaminopurine (BAP) and 2,4- Dichlorophenoxyacetic Acid (2,4-D) Concentrations. Jurnal Sains dan Seni Pomits, 2: 1-6.
  11. Santoso, U. & Nursandi, F. (2004). Plant tissue culture. Universitas Muhammadiyah Malang Press. Malang.
  12. Andaryani, S. (2010). Study different concentration of BAP and 2,4-D on in vitro callus induction of Jatrophacurcasathesis. Sebelas Maret University, Surakarta.
  13. Arianti, S. N., Wacniati., , dan Suwastika, I. N. (2012). Callus induction of Theobroma cacao L. on? MS Media with addition of 2,4-D, BAP and coconut water. Jurnal Natural Sciences 1(1) : 74-88.
  14. Suaib dan Gusti, R. S. (2014). Plant tissue culture. Kendari : Solo Printing.
  15. Lestari, E.G. (2011). The role of growth regulators in plant multiplication by tissue culture. Jurnal Agro Biogen 7(1): 63-68.
  16. Winarto, B., Matjik N. A., Purwito, A dan Marwoto, B. (2010).? Application of 2,4-D and TDZ on formation and callus regeneration in anther culture of Anthurium.? Jurnal Hortikultura, 20 (1):1-9.
  17. (2014). In vitro Callus induction of jasmine leaves (Jasminumsambac) by addition of different concentration of DichlorophenoxyaceticAcid (2,4-D) and 6-Benzylamino Purine ?(BAP) on MS media. Universitas Negeri Surabaya. Jurnal Lentera Bio Vol. 3 No. 3.

[Ayu Yusna, Fauziyah Harahap and Syahmi Edi. (2018); EFFECT OF PLANT GROWTH REGULATORS ON IN VITRO CALLUS INDUCTION OF SHOOT EXPLANT MANGOSTEEN (Garcinia mangostana L.) Int. J. of Adv. Res. 6 (11). 123-129] (ISSN 2320-5407). www.journalijar.com


Fauziyah Harahap
Lecture of Postgraduate Biology Education, State University of Medan, Medan, Indonesia

DOI:


Article DOI: 10.21474/IJAR01/7978       DOI URL: http://dx.doi.org/10.21474/IJAR01/7978


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