01Mar 2019


  • Ministry of Science and Technology, Treatment and disposing of chemical, biological and military hazardous wastes, Center for research and development, Baghdad ? Iraq.
Crossref Cited-by Linking logo
  • Abstract
  • Keywords
  • References
  • Cite This Article as
  • Corresponding Author

The aim of this study is to isolate and identify Species of blood-contaminated bacteria in Blood bank refrigerators which they are out of service for several reasons can be diagnosis and identification via Five samples (Swabs) which they are prepared from inside of leftedrefrigerator at the stores of the private nursing hospital at the city of Baghdad, samples are cultured on blood agar media, after 24 hours, results showed three species of bacteria, these bacteria are diagnosed via biochemical and enzyme-based tests according to Bergey\'s Manual 2005, which they are Staphylococcus aureus, Bacillus cereus,andEnterococcus faecalis. These species are diagnosed because of their ability to resist environmental conditions for a long time. There are other species of bacteria are not being diagnosed because of inability to resist dryness or changing of temperature degrees or sterilization.

  1. Carson, J.L., Stanworth, S.J., Roubinian, N., Fergusson, D.A., Triulzi, D., Doree, C., Hebert, P. C., 2016. Transfusion thresholds and other strategies for guiding. allogeneic red blood cell transfusion.?The Cochrane Database of Systematic Reviews. Cochrane library. Wiley 1-24.
  2. Ibrahim, I.H., 2013. Pathogenic bacteria, Chapter 5. The genus of Staphylococcus, first edition 50-61
  3. Masalha, M., Borovok, I., Schreiber, R., Aharonowitz, Y., Cohen, G., 2001. Analysis of transcription of the Staphylococcus aureus aerobic class Ib and anaerobic class III ribonucleotide reductase genes in response to oxygen 183(24),7260-72.
  4. Tong, S.Y., Davis J.S., Eichenberger, E,. Holland, T.L,. Fowler, V.G., 2015. \"Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management\". Clinical Microbiology Reviews 28
  5. Wade Road., 1982. Agents and Main Distributors 3th ed. Oxoid 88-100.
  6. Senok, A.C., Verstraelen, H., Temmerman, M., Botta, G A., Senok, A.C., 2009. Probiotics for the treatment of bacterial vaginosis. The Cochrane Database of Systematic Reviews. Cochrane library. Wiley 1-24.
  7. Hoffman, B., 2012. Williams gynecology, 2nd edition. New York: McGraw-Hill Medical. 65
  8. Valyal, B., Das, S.K.R., Kavya, T., Vidyasagar, V., Begum, R., 2017. Antibiotic sensitivity and resistance pattern in orthopedic infectious cases of a tertiary care teaching hospital. Pharmacology. 7 (4), 24- 29
  9. Sanford, C.A., Jong, E.C., 2008.?The Travel and Tropical Medicine Manual. Elsevier Health Sciences?469
  10. Asaeda, G., Caicedo, G., Swanson, C., 2005. Fried Rice Syndrome.?Journal of Emergency Medical Services.?30?(12), 30?32.
  11. Guinebreti?re, M.H., Auger, S., Galleron, N., Contzen, M., De Sarrau, B., De Buyser, M.L., Lamberet, G., Fagerlund, A., Granum, Per E., Lereclus, D., De Vos, P., Nguyen-The, C., , A., 2013.?Bacillus cytotoxicus sp. nov. is a novel thermotolerant species of the Bacillus cereus Group occasionally associated with food poisoning.?International Journal of Systematic and Evolutionary Microbiology.?63?(1), 31?40.
  12. Frankland, G.C., Frankland, P. F., 1887. Studies on Some New Micro-Organisms Obtained from Air.?Philosophical Transactions of the Royal Society B: Biological Sciences.?178, 257?287.
  13. Kocur, M., Martinec, T., 1959. The Taxonomic Study of Sarcina Aurantiaca Fluegge. International Bulletin of Bacteriological Nomenclature and Taxonomy 9 (4), 165-168
  14. Sandle, T., 2014.?The Risk of?Bacillus cereus?to Pharmaceutical Manufacturing.?American Pharmaceutical Review?(Paper).?17?(6): 56.
  15. Alseth, I., Rognes, T., Lindb?ck, T., Solberg, I., Robertsen, K., Kristiansen, K.I., Mainieri, D., Lillehagen, L., Kolst?, A-B., Bj?r?s, M., 2006.?A new protein superfamily includes two novel 3-methyladenine DNA glycosylases?Bacillus cereus, AlkC and AlkD.?Molecular Microbiology 59
  16. Bories, G., Brantom, P., de Barber?, Joaquim Brufau; et al. (2008).?Safety and efficacy of the product Toyocerin (Bacillus cereus var. toyoi) as feed additive for rabbit breeding does.?EFSA Journal. Scientific Opinion of the Panel on Additives and Products or Substances used in Animal Feed.?European Food Safety Authority.??913: 1-13
  17. Alksne, LE., Projan, SJ. (2000). Bacterial virulence as a target for antimicrobial chemotherapy. Curr Opin Biotechnol. 11: 625?636.
  18. Andrup, L., Andersen, K. (1999). A comparison of the kinetics of plasmid transfer in the conjugation systems encoded by the F plasmid from Escherichia coli and plasmid pCF10 from Enterococcus Microbiology 145, 2001?2009.
  19. Bearman, G ML., Wenzel, RP. (2005). Bacteraemias: a leading cause of death. Arch Med Res 36: 646?659.
  20. Billstr?m, H., Lund, B., Sullivan, A?. & Nord, C. E. (2008). Virulence and antimicrobial resistance in clinical Enterococcus faecium. Int J Antimicrob Agents 32: 374?377.
  21. Brown, D. F. J., Brown, N. M., Cookson, B. D., Duckworth, G., Farrington, M., French, G. L., King, L., Lewis, D., Livermore, D. M. & other authors (2006). National glycopeptide-resistant enterococcal bacteremia surveillance Working Group report to the Department of Health. J. Hosp Infect 62 (Suppl. 1), 1?27.
  22. Budzik, J M., Schneewind, O. (2006). Pili prove pertinent to enterococcal endocarditis. J Clin Invest 116, 2582?2584.
  23. Busani, L., Del Grosso, M., Paladini, C., Graziani, C., Pantosti, A., Biavasco, F., Caprioli, A. (2004). Antimicrobial susceptibility of vancomycin-susceptible and -resistant enterococci isolated in Italy from raw meat products, farm animals, and human infections. Int J Food Microbiol. 97
  24. MOH, (2013).Standard Operation Procedure for Facility and Equipment Maintenance Maintenance Management in primary Health Care Centers.P18.
  25. Berk SL, Verghese A. (1989) Emerging pathogens in nosocomial pneumonia.?Eur J Clin Microbiol Infect Dis. 8(1):11?14

[Rasha Kifah Hassan, Ahmed Ibrahim Jessim, Ammar Salim Salman and Abbas Fadhil Salman. (2019); ISOLATION AND IDENTIFICATION OF BACTERIAL CONTENT IN BLOOD BANK REFRIGERATORS. Int. J. of Adv. Res. 7 (3). 60-65] (ISSN 2320-5407). www.journalijar.com

Ahmed Ibrahim Jessim
Ministry of Science and Technology, Treatment and disposing of chemical, biological and military hazardous wastes, Center for research and development, Baghdad – Iraq


Article DOI: 10.21474/IJAR01/8599       DOI URL: http://dx.doi.org/10.21474/IJAR01/8599

Share this article