24Dec 2019

GENETIC VARIATION IN A JATROPHA CURCAS JATROPHA INTEGERRIMA HYBRID

  • Centro de Investigacion y Asistencia en Tecnologia y Diseno del Estado de Jalisco A. C. Subsede Sureste. Tablaje Catastral 31264 Km 5.5 Carretera Sierra Papacal - Chuburna Puerto Parque Cientifico Tecnologico de Yucatan CP: 97302 Merida, Yucatan, Mexico
  • Universidad Autonoma de Yucatan, Facultad de Ingenieria Quimica, Periferico norte, Km. 33.5, Tablaje catastral 13615, Chuburna de Hidalgo Inn, 97203 Merida, Yucatan, Mexico
  • Campo Experimental Chetumal, INIFAP, Carretera Chetumal-Bacalar, Km 25, Chetumal, Quintana Roo, Mexico. C.P. 77900
  • Departamento de Biotecnologia y Bioquimica. Centro de Investigacion y de Estudios Avanzados del IPN, Unidad Irapuato. Irapuato, Guanajato., CP. 36821. Mexico
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A viable hybrid was produced by an interspecific Jatropha curcas x Jatropha integerrima cross. Artificial cross pollination was used, with J. integerrima being the pollen donor for J. curcas. Hybrid fruit resulted. Seeds from this fruit were germinated in vitro to produce seedlings as a source of leaf explants for genomic DNA extraction and purification. Molecular markers (ISSR and DAMD) were used to confirm hybridization success and characterize the combination of parental characteristics in the hybrid. Based on the band pattern, this interspecific cross resulted in genetic variation in the hybrids DNA, since 15% of its DNA content exhibited no similarity to either parent genome. Of the remaining DNA content, 35% was similar to the female parent (J. curcas), 25% to the male parent (J. integerrima), and 25% was a combination of both parents.

  1. Aguilera-Cauich, E. A., Perez-Brito, D., Yabur, A. N., Lopez-Puc, G., Najera, G. C., Rivero, J. C. S., Mijangos-Cortes, J. O. (2015). Assessment of phenotypic diversity and agronomic contrast in American accessions of Jatropha curcas L. Industrial Crops and Products, 77, 1001?1003. https://doi.org/10.1016/j.indcrop.2015.09.063
  2. Hu, J., Bin, Li, J. W., Wang, L. J., Liu, L. J., & Si, S. W. (2011). Utilization of a set of high-polymorphism DAMD markers for genetic analysis of a cucumber germplasm collection. Acta Physiologiae Plantarum, 33(1), 227?231. https://doi.org/10.1007/s11738-010-0525-7.
  3. Ince, A. G., Karaca, M., & Onus, A. N. (2009). Development and utilization of diagnostic DAMD-PCR markers for Capsicum accessions. Genetic Resources and Crop Evolution, 56(2), 211?221. https://doi.org/10.1007/s10722-008-9356-4.
  4. Isshiki, S., Iwata, N., & Khan, M. M. R. (2008). ISSR variations in eggplant (Solanum melongena L.) and related Solanum species. Scientia Horticulturae, 117(3), 186?190. https://doi.org/10.1016/j.scienta.2008.04.003
  5. Kumar, R. S., Parthiban, K. T., Hemalatha, P., Kalaiselvi, T., & Rao, M. G. (2009). Investigation on cross-compatibility barriers in the biofuel crop Jatropha curcas L. with wild Jatropha species. Crop science, 49(5), 1667-1674.
  6. Murashige, T., Skoog, F. (1962): A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15:473-497.
  7. One, K. T., Muakrong, N., Phetcharat, C., Tanya, P., & Srinives, P. (2014). Inheritance of dwarfiness and erect growth habit in progenies of Jatropha curcas? Jatropha integerrima. Journal of the American Society for Horticultural Science, 139(5), 582-586.
  8. Sahai, K., Kumar, S., & Rawat, K. K. (2009). Floral abnormalities in Jatropha tanjorensis Ellis & Saroja (Euphorbiaceae): A natural interspecific sterile hybrid. Plant Species Biology, 24(2), 115?119. https://doi.org/10.1111/j.1442-1984.2009.00245.x
  9. Subashini, G., Paramathma, M., & Manivannan, N. (2015). Character association analysis for oil yield and yield components in bc4 f1 population of interspecific cross ( Jatropha curcas x Jatropha integerrima ). SABRAO Journal of Breeding and Genetics, 47(4), 335?339.
  10. Tanya, P., Taeprayoon, P., Hadkam, Y., & Srinives, P. (2011). Genetic Diversity Among Jatropha and Jatropha-Related Species Based on ISSR Markers. Plant Molecular Biology Reporter, 29(1), 252?264. https://doi.org/10.1007/s11105-010-0220-2
  11. Zhou, Z., Bebeli, P. J., Somers, D. J., & Gustafson, J. P. (1997). Direct amplification of minisatellite-region DNA with VNTR core sequences in the genus Oryza. Theoretical and Applied.

[Latour - Gordillo Juan Jorge, Aguilera - Cauich Erick Alberto, Herrera - Cool Gilbert Jose, Mellado -Mojica Erika, Uc - Varguez Alberto, Barba - Gonzalez Rodrigo and Lopez - Puc Guadalupe (2019); GENETIC VARIATION IN A JATROPHA CURCAS JATROPHA INTEGERRIMA HYBRID Int. J. of Adv. Res. 7 (Dec). 136-143] (ISSN 2320-5407). www.journalijar.com

Guadalupe Lopez Puc
Centro de investigacion y asistencia en tecnologia y diseño del estado de Jalisco AC

DOI:

Article DOI: 10.21474/IJAR01/10137      
DOI URL: http://dx.doi.org/10.21474/IJAR01/10137

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