20Jan 2020

PROTECTIVE EFFECT OF BETA VULGARIS ON CARBON TETRACHLORIDE INDUCED HEPATOTOXICITY IN ADULT WISTAR RAT

  • Abia State University, Uturu, Nigeria
  • Howard University, Washington DC, U.S.A
  • Enugu State University of Science and Technology, Nigeria
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Beta vulgaris also known as beet root has been used to treat a wide range of symptoms and diseases, such as fever, constipation, cancer, diabetes, liver and heart diseases. The aim of this study was to investigate the protective ability of beta vulgaris on the liver following Carbon tetrachloride (CCl4) intoxication. Twenty five adult albino wistar weighing between 150g and 200g were used in this study. The animals were divided into five groups A, B, C, D, and E. Groups A and B served as control were injected with paraffin oil for two consecutive days and group B fed with Beta vulgaris extract alone. Groups C and D were injected with CCl4 (1.0ml/kg b.w i.p) for two consecutive days and group D fed with BE throughout the experimental days. Group E was fed with Beta vulgaris extract throughout the experiment and injected CCl4 on the last two days of the experimental period (28 days). At the end of the experimental period animals were reweighed and sacrificed using chloroform vapour. Blood was collected for serum enzyme analysis and liver was harvested and trimmed for histological studies. The result showed that, there was a decrease in mean body weight of 5% in group C animals while there was an increase in other groups. The liver weight were significantly higher in group C (p<0.05) when compared with control groups and other experimental groups. The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) in group C were significantly higher (p<0.05) when compared with the control group while groups B, D and E were normal. Histological examination of liver (H&E) showed numerous fatty changes, distortion of hepatocytes, focal necrosis, loss of nuclei and cytoplasmic shrinkage in group C animals, groups A, B and E appear normal, while group D showed mild focal inflammation and areas of regeneration of tissues. These results shows that Beta vulgaris extract has the ability to prevent and also restore histological and biochemical changes in the liver following CCl4 injection.


  1. Chevallier A., (1996). The Encyclopedia of Medicinal Plants. New York: DK Publishing Inc.; 176.
  2. Doherty RE (2000). \"A History of the Production and Use of Carbon Tetrachloride, Tetrachloroethylene, Trichloroethylene and 1,1,1-Trichloroethane in the United States: Part 1?Historical Background; Carbon Tetrachloride and Tetrachloroethylene\". Environmental Forensics. 1 (1): 69?81. doi:1006/enfo.2000.0010.
  3. Kujala TS, Vienola MS, Klika KD, et al. (2000). Betalain and phenolic compositions of four beetroot (Beta vulgaris) cultivars. Eur Food Res Tech 214, 505?510
  4. Masuda Y (October 2006). \"[Learning toxicology from carbon tetrachloride-induced hepatotoxicity]\". Yakugaku Zasshi (in Japanese). 126 (10): 885?99. doi:1248/yakushi.126.885. PMID17016019.
  5. Recknagel RO (1967). \"Carbon tetrachloride hepatotoxicity\". Pharmacol. Rev. 19 (2): 145?208. PMID4859860.
  6. Rood AS, McGavran PD, Aanenson JW, Till JE (2001). \"Stochastic estimates of exposure and cancer risk from carbon tetrachloride released to the air from the rocky flats plant\". Risk Anal. 21 (4): 675?95. doi:1111/0272-4332.214143. PMID11726020.
  7. Seifert WF, Bosma A, Brouwer A, et al. (1994). \"Vitamin A deficiency potentiates carbon tetrachloride-induced liver fibrosis in rats\". Hepatology. 19 (1): 193?201. doi:1002/hep.1840190129. PMID8276355.
  8. Sener G, Sa?an ?, Yanardag R, Ayanoglu-D?lger G (2002). Effects of chard (Beta vulgaris L. var. cicla) extract on oxidative injury in the aorta and heart of streptozotocin-diabetic rats. Journal of medicinal food 5: 37?42.
  9. Thnaian Althnaia, Ibrahim Albokhadaim, Sabry M El-Bahr, (2013). Biochemical and histopathological study in rats intoxicated with carbon tetrachloride and treated with camel milk. Springerplus journal, doi: 10.1186/2193-1801-2-57.

[Solomon Egeonu C, Hope Okechukwu K, Churchil Ihentuge J, Godwin Ndukwe U and Chike Anibeze I.P (2020); PROTECTIVE EFFECT OF BETA VULGARIS ON CARBON TETRACHLORIDE INDUCED HEPATOTOXICITY IN ADULT WISTAR RAT Int. J. of Adv. Res. 8 (1). 209-217] (ISSN 2320-5407). www.journalijar.com


Solomon Egeonu Chimaraoke
Abia State University, Uturu, Nigeria

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Article DOI: 10.21474/IJAR01/10285       DOI URL: http://dx.doi.org/10.21474/IJAR01/10285


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