Silver Nanoparticles Produced by Some Enteric Bacteria from Chronic Rhinosinusitis

Thirty five sinus samples obtained from Endoscopic patients who were suffering from chronic sinusitis in Mosul Republic hospital in Mosul city from March 2013 to March 2014 were conducted .Samples were cultured and pure isolates were identified to species level using morphological, biochemical and physiological tests. The results indicate that Klebsiella pneumoniae which was isolate at (13.3 %) ,one isolate for each of Citrobacter freundii, Moraxella spp.,Serratia marcescens, Staphylococcus capitis,Staph. lugdunensis,Staph. saprophyticus and Staph. sciuri at (6.6%) all from males samples . Escherichia coli at (15%) ,Acinetobacter lwoffii, Proteus mirabilis ,Enterobacter aerogenes,Enterobacter cloacae, Serratia marcescens, Staph.epidermidis, Staph.hominis, Staph.warneri and Staph.xylosus at (5%) all from females sampels. All isolates were completely resistant to Ampicillin25 , Augmantin(Amoxicillin + Clavulanic acid)30 ,Ampiclox (Ampicillin+Cloxacillin)30, Amoxicillin25, Erythromycin15, Meropenem10, and Carpencillin25.Isoletes give high resistance to Cefotaxime10 and Cephalothin30 at (95.24%), they were slightly resistant to Amikacin10, Gentamicin10 and Imipenem10 at ( 9.52%). Finally all isolates were sensitive to Ciprofloxacin10 and Norfloxacin10 which were resistant to high stable active silver colloidal nanoparticle(NP) 10 ppm.Detection of some virulence factors such as urease, hemolysin, ,protease,gelatinase and lipase. Biosynthesis of silver nanoparticles was investigated in two isolates E.coli and Kleb. pneumoniae , the extracellular synthesis of AgNPS was initially detected by visual inspection of culture flask for a change in the color of cultural medium from clear to brown/green.