Bacteriological and genetic study on Escherichia coli Causing Acute cholecystitis calculus for Diabetes patients in AL-Diwanyia City

The aim of this study is identify the E.coli bacteria that cause acute cholecystitis calculus for patients with diabetes in the AL- Diwaniya city, genetically. The study include 110 patients (97 female, 13 male) undergoing cholecystectomy at the general Diwaniya Teaching Hospital in from November 2013 to April 2014 .from each patien ,samples bile, a sample of the tissue of the gallbladder, and gall stone( if present).All samples were cultured on different media for full bacteriological identification The results showed that chronic cholecystitis is the most common ratio of 55.4%, followed by acute cholecystitis as the form of 44.6%. It is noted in this study that the age and gender effect on infection of bitterness as the highest injury rate recorded at the 45-36 year group among both sexes, and in the distribution of pathological cases, according to the sex of the patient turns out that there are greatly significant proportions infection bitterness between females and males reaching injury females 88.1%, while the injured were males 11.9% .The results showed that 41.5% of the total samples gave positive-growing bacteria on the different media .The bitterness of the fabric of bacterial isolation was 52%, and the bile 26.4%, and 21.6% gall stone. isolated E.coli bacteria were of the fabric of the gallbladder 40%, then the bile was 30.3%, and then gall stone 29.7%. Has been investigating the viability of these isolates to produce enzymes CTX-M, SHV, TEM and Ampc broad- spectrum ?- lactames enzymes by revealing the presence of genes bla-Tem ,bla-CTX-M, bla-SHV and bla-Ampc among those isolates by using The polymerization chain reaction (PCR) It showed 23/26 (88.4%) isolates contained the bla-Ampc gene, the containment of 7/26 (26.9%) the isolates of the bla-SHV gene , and the containment of 18/26 (69.2%) the isolation of the bla-Tem gene, while 20/26 (76.9%),from the isolation of the containing bla-CTX-M gene.