New Delhi Metallo-beta-Lactamase 1 (NDM-1) Producing Acinetobacterbaumannii in Egyptian Hospitals.

Resistance to carbapenems is developing around the world.Acinetobacterbaumannii( A. baumannii) resistance to carbapenems is associated with production of carbapenem-hydrolyzing class B metallo-?-lactamases (MBL).New Delhi metallo-?-lactamase 1 (NDM-1) is one of the most recently discovered MBL among A. baumannii. Aim: The present work was aimed to study the prevalence of bla NDM-1 gene amongcarbapenem-resistantA. baumanii isolates from two hospitals inBenha, Egypt. Materials and Method:40 A. baumanii clinical isolates were investigated for different antibiotics and imipenem resistant using the standard disk diffusion method.Combination disc Test (CDT), Double Disc Synergy Test (DDST) and Epsilometer test(E test) were performed for phenotypic detection of MBLs and the molecular method polymerase chain reaction (PCR) was done for detection of NDM-1gene. Results: Out of 40 imipenem resistantA. baumanii 62.5%,55% and65% of them had positive CDT,DDST and E test respectively. NDM-1 gene was detected in 5(19.2%) A. baumanii MBL producing isolates by molecular method. Conclusion:bla NDM-1 production contributes to carbapenem resistance in A. baumannii in Egypt and highlights the need to screen and detect this enzyme to prevent and control their dissemination.