Biochemical and Molecular Study in Women with Diabetes Mellitus Type 1

Abstract: Diabetes is a chronic metabolic syndrome resulting from defects in insulin secretion or action or both; The aim of this study was to detecting single strand antibodies against DNA in Type 1 Diabetes mellitus DM patients with nephropathy and myocardial vascular disease complication. The results showed that a significant increase in levels of Fasting blood glucose, Urea and BUN, lipid Prfile except HDL levels compared with control groups. Also, the study showed the purity of extracted DNA from blood and placenta in control groups and Placenta was higher than of D.M groups. Electrophoresis pattern showed that band of native DNA was more distinct and intense as compared with DM groups that migrated as short band of lower fluorescence intensity form indicating structural alterations in the DNA upon glycation. In this study, generation of single strand breaks has been confirmed by nuclease S1 digestion studies. The results showed a substantial digestion of modified DNA in DM groups by S1-nuclease while native DNA remained unaffected. The comet assay revealed higher percentage of DNA damage in diabetics groups when compared to control groups. The damage indicated by tail and head of comet, the study showed an increase in tail comet and a decrease in comet head in D.M patients with and without complications compared with control group.

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[Saad Mohammed Nida, Israa B. Raoof, Perry H. Saifullah (2015); Biochemical and Molecular Study in Women with Diabetes Mellitus Type 1 Int. J. of Adv. Res. 3 (4). 0] (ISSN 2320-5407).

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