ENDOSULFAN TOLERATE / DEGRADING BACTERIA ISOLATED FROM COTTON FIELD SOIL

In this study endosulfan tolerate/degrading bacteria isolated from endosulfan-polluted Gossypium arboretum L rhizosphere soil, through repetitive enrichment culture method. The endosulfan was detection by TLC, Spectrophotometric and GC-MS. The TLC and GC-MS chromatography revealed that ? - endosulfan, ? - endosulfan and endosulfan diol. Therefore, a higher level of endosulfan residues was presented in Gossypium arboreum L rhizosphere soil of Tamilnadu, India. Four different bacteria were isolated from enrichment soil sample. This method proved successful in yielding the culture, ISB9 with considerably high potential to tolerate/degrade endosulfan (200 mg L-1). The bacterial isolate of Chromobacterium violaceum (ISB9) culture was able to degrade endosulfan to endosulfan diol which is nontoxic as compared to the parent compound.