INHIBITION OF HISTAMINE FORMATION ON THE FRIGATE TUNA (Auxis thazard thazard, L) USING LEAF EXTRACT OF Jatropha curcas

The study of the inhibition of histamine formation on the frigate tuna using jatropha leaf extract has been done. Jatropha leaf macerated in stages ranging from hexane, chloroform, ethyl acetate and methanol. Results of preliminary research note that the ethyl acetate extract and methanol with a concentration of 1% (w / v) is the best extract that provides antibacterial activity against several of histamine-forming bacteria. In this experiment, frigate tuna were divided into 3 groups, group 1 was soaked for 30 minutes in water, group 2 soaked for 30 minutes in a solution of ethyl acetate extract of leaf jatropha with a concentration of 1%, and group 3 was soaked for 30 minutes in a solution of methanol extract of leaf jatropha with a concentration of 1%. After that, frigate tuna stored at room temperature for 5 hours, then analyzed the number of bacterial colony with total plate count method (TPC), number of histamine-forming bacteria and histamine levels. The results showed that the value of TPC the frigate tuna its soaked in water are log 6.68 (cfu / g), soaked in a solution of ethyl acetate extract are log 4.94 (cfu / g) and soaked in a solution of methanol extract are log 5.61 (cfu / g). The number of histamine-forming bacteria in the frigate tuna its soaked in water are log 5.62 (cfu / g), soaked in a solution of ethyl acetate extract are log 4.40 (cfu / g) and soaked in a solution of methanol extract are log 5.54 (cfu / g). histamine levels of the frigate tuna its soaked in water are 17.73 mg / kg, soaked in a solution of ethyl acetate extract are 11.07 mg / kg and soaked in a solution of methanol extract are 14.02 mg / kg. Thus, the ethyl acetate extract of leaves of Jatropha is more effective to inhibiting the growth of histamine-forming bacteria and inhibit the formation of histamine in fresh frigate tuna that stored for 5 hours at room temperature.