Isolation of bacteriophages from wastewater for possible utilization to combat multi-drug resistant Pseudomonas spp. isolated from public water pool.
- BioEra Life Sciences Pvt. Ltd., BioEra Park, S. No. 125, Mumbai-Bangalore Highway, Tathawade, Pune – 411033, Maharashtra, India.
- Alakaa High School, Ministry of Education, Thiqar, Suq al Shiuk, Iraq.
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Abstract
A multi drug resistant Pseudomonas spp. was isolated and characterized from public water pools. In order to develop combat strategy to eradicate and prevent this opportunistic pathogen, bacteriophages were isolated from wastewater samples. Isolated bacteriophage showed large clear plaques indicating presence of lytic phages with higher infectivity and burst size of 36 x 107 PFU/ml. Genome size of the bacteriophage was approximately 30675 bp with 10 fragments yielded from EcoRI restriction and 6 fragments from HindIII restriction. Proteome analysis showed 10 well separated bands on SDS PAGE.
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Asha Salunke, Hemal Makani, Archana Rasure and Wisam Abdulkareem Hussein. (2016); Isolation of bacteriophages from wastewater for possible utilization to combat multi-drug resistant Pseudomonas spp. isolated from public water pool., International Journal of Advanced Research (IJAR), 4 (07), 633-640, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/923
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