AUXIN AND CYTOKINE TREATMENT EFFECT IN COMBINATION WITH SUCROSE ON IN VITRO POTATO REGENERATION.

In this study we focused on in vitro potato regeneration under different hormonal (auxin/cytokine) treatment with combination of sucrose in three potato varieties, “Nevsky, Riviera, Zefira”. Three parameters were observed in response to treatment, number of shoots development, size of shoots, and root length. In vitro tissue culture propagation of three potato cultivars (Solanum tuberosumL.) “Nevsky”, Zefira” and “Riviera” were studied on modified MS medium supplemented with hormones (IBA/BAP) concentration: MS + 30g/l sucrose (6% MS medium), 6%MS medium +1mg/l BAP + 0,05mg/l IBA and 6% MS medium +1mg/l BAP + 0,1mg/l IBA. Morphological characterization of all three in vitro potato cultivars on modified MS medium was variable depending on varieties.It was revealed that high concentration of IBA has negative effect on plants development, respectively 6% MS medium + 1mg/l BA + 0,1mg/l IBA was not optimal neither cultivars of potato. According to the results cultivars Zefira” and “Riviera” had maximum potential for in vitro rooting ( correspondingly: 82.97% and 100%) and shoots (100% and 87.34%) formation on 6% MS medium + 1mg/l BA + 0,05mg/l IBA, but “Nevsky” gave maximum development (rooting-82% and shoot formation -87%) on 6% MS medium.