The Plackett-Burman Model-Optimization of Significant Nutritional Parameters for Petroleum Bioremediation by Pseudomonas sp.

It is worthwhile to mention that Plackett-Burman design is useful not only in evaluating the significant of some variables on the bioprocess, but also in comparing between different categories. Hence maintain a comprehensive evolution of the overall process. In this study Plackett-Burman experimental design had been applied to evaluate the significant of cultural condition affecting petroleum bioremediation by isolated pseudomonas sp, seven variables through eight trials were studied simultaneously, namely Temperature, pH, Peptone, KH2PO4, K2HPO4, NaH2PO4, Na2HPO4 to elucidate their effect . Based on bacterial growth by taking O.D at 600 nm, Temperature, Na2HPO4 were recognized as the most effective factors. The regression coefficient R2 (0.99) ensure the adequate integrity of the model. The enhanced growth capability with appropriate cultural condition in presence of only signal carbon source as petrol of Pseudomonas sp. by optimizing the tools suggests a suitable method not only in evaluating the significance of some variables but also in comparing between different categories, in which was difficult to compare between their effects in conventional experiments, and hence maintain a comprehensive evaluation of the overall process.