The Effect of Deposition Plasma Current For Gold Nano Thin Film on Bacteria Using Cold Plasma Sputter

New and special physical mechanism for Synthesizing Gold Nano particles purely and directly was used to deposit Gold Nano particles directly on a bacteria surface to study the effect of the Gold particles in Nano scales with changes in deposition current of plasma sputter (discharge current) on the bacterial behavior according to the effect of antibiotic, pH and temperature on the bacteria and compare it to the effect of the Gold without using chemical methods or assisting agents (catalysis) in Synthesis of them. For this purpose, two types of bacteria have been selected, gram negative Escherichia coli (E.coli), and gram positive Staphylococcus aureus. (12) Types of antibiotics was used, it was found that the bacterial sensitivity to the antibiotics changes by Gold deposition rate, and every antibiotic have its own effect. Noted that Gold deposition in nano scale on bacteria decrease bacterial activity when grown in different pH rate and temperature. Concluding that Gold nano particle have marked effect on bacteria without causing changes in the size of the bacterial cell, and bacterial sensitivity variable to each antibiotic particularly.