IMMUNOGENICITY OF SALIVARY GLAND AND MIDGUT HOMOGENATE ANTIGENS AGAINST CAMEL TICK HYALOMMA DROMEDARII (IXODOIDEA: IXODIDAE) AND RELATED BIOLOGICAL IMPACTS AND CONTROL POTENTIAL : IN VIVO STUDY.
Walaa Ahmed Moselhy
, Aly Fahmy El-Sayed
, Fatma El-Sayed Ali
, Eman Ahmed El-Kelesh
, Wesam Soliman Ebrahim
and Abd El-Baset Badr El-Din Zayed.
- Zoology Department- Faculty of Science - Al-Azhar University (Girls branch). \ VACSERA- Egypt.
- Parasitology Department, Animal Health Research Institute, Dokki, Giza, Egypt. \
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Salivary gland (SgAg) and midgut (MgAg) antigens derived from partially fed female H. dromedarii were used to vaccinate rabbits at a dose of 100 µg/kg protein. The immunized hosts were challenged with unfed female ticks. Data revealed a significantly decreased feeding percent and duration, engorged tick and egg mass weights, number of eggs laid, hatching percent and fertility compared to control groups. Overall vaccine efficacy was evaluated as 67% and 43.79% for SgAg and MgAg, respectively, that means tick Ags used induced best protection against H. dromedarii population. Immunogenicity of experimental vaccine homogenates revealed a strong and specific immune response against both antigens. Western blot analysis revealed that 7 polypeptide bands ranging in their molecular weights from 95.8 to 32.3 kDa were recognized in SgAg, whereas 10 polypeptide bands ranging from 93.7 to 8.6 kDa were identified in MgAg.
[Walaa Ahmed Moselhy, Aly Fahmy El-Sayed, Fatma El-Sayed Ali, Eman Ahmed El-Kelesh, Wesam Soliman Ebrahim and Abd El-Baset Badr El-Din Zayed. (2016); IMMUNOGENICITY OF SALIVARY GLAND AND MIDGUT HOMOGENATE ANTIGENS AGAINST CAMEL TICK HYALOMMA DROMEDARII (IXODOIDEA: IXODIDAE) AND RELATED BIOLOGICAL IMPACTS AND CONTROL POTENTIAL : IN VIVO STUDY. Int. J. of Adv. Res. 4 (Aug). 2139-2149] (ISSN 2320-5407). www.journalijar.com
