SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES USING BLEPHARIS MADERASPATENSIS (L) HYNE EX ROTH LEAF EXTRACT AND IT’S ANTI- INFLAMMATORY ACTIVITY AGAINST HUMAN BLOOD CELLS.

In this current study green synthesis of silver nanoparticles using aqueous leaf extract of blepharis maderaspatensis and its anti – inflammatory activities are evaluated. The anticipation of hypo tonicity induced HRBC membrane lysis was taken as an appraise of the anti – inflammatory activity. Results revealed that synthesized silver nanoparticles from the leaf extract of blepharis maderaspatensis hyne Ex.roth showed strong absorption maximum at 400nm corresponding to the surface plasma resonance. Green synthesis of silver nanoparticles was characterized by UV-Visible spectroscopy, SEM, FTIR, XRD and Zeta potential. The size of the nanoparticles was found to be >100nm by using XRD and SEM analysis. It is a well documented fact that the silver nanoparticles synthesized from blepharis maderaspatensis hyne ex Roth leaf extract showed effective anti- inflammatory activity against human blood cells. Therefore these herbal extracts could be used in future as alternative therapeutic agents for the treatment of human diseases.