IN VITRO PROPAGATION AND PRODUCTION OF TEPHROSIA PURPUREA PLANT VIA INTERNODAL EXPLANTS.

Tephrosia purpurea (Linn.) Pers. (Leguminasae), is a highly suberect, branched and herbaceous perennial herb. According to Ayurveda survey of literature, this plant is extensively used in the treatment of all types of wound healing diseases. The present study was framed out to develop the protocol for the in vitro propagation via intermodal cultures of Tephrosia purpurea. Initially the nodal explants were inoculated on the MS medium supplemented with various concentrations of BAP and KN (2.0-5.0). The initiation of roots and its elongation was achieved using various concentrations of IAA and NAA in combination. The highest number of shoots and shoot length 22 and 7.15± 1.1was found at 3.4 mg/L of KN. The highest number of roots and root length 28 and 8.09 ±0.1 was observed at 5.0+1.1 mg/L of IAA and NAA. By the current studies we conclude that KN was found more effective in the proliferation of shoot buds comparing to the BAP.