HPTLC DETERMINATION OF CILOSTAZOL IN PHARMACEUTICAL DOSAGE FORMS

A simple, selective and precise high performance thin layer chromatographic method was developed and validated for the determination of Cilostazol in bulk drug and in formulation. The method uses aluminium plates pre-coated with silica gel 60F-254 as the stationary phase and hexane:acetone:chloroform (5:2:3, v/v/v) as solvent system. This system gave compact spot for Cilostazol (Rf: 0.15 ± 0.01). Densitometric analysis of Cilostazol was performed in the absorbance mode at 254nm. The linear regression analysis data for the calibration plot showed good linear relationship over a concentration range of 1 – 10 µg spot-1. The values of correlation coefficient, slope and intercept were 0.998, 1489 and 4915 respectively. The method was validated for precision, robustness and recovery. The limit of detection and limit of quantification were 0.089 and 0.269µg spot-1, respectively.