EFFECT OF SINGLE NEOCLEOTIDE POLYMORPHISM OF RECK GENE PROMOTER ON HEPATOCELLULAR CARCINOMA: IN CORRELATION WITH CLINICAL AND LABORATORY PARAMETERS.

Background:-Hepatocellular carcinoma (HCC) is the second most common cancer in men and the 6th most common cancer in women in Egypt. It constitutes 70.48% of all liver tumors among Egyptians. Thereversion-inducing-cysteine-rich protein with Kazal motifs (RECK) gene encodes a membrane-anchored glycoprotein that can negatively regulate matrix metalloproteinases (MMPs) and inhibit tumor invasion, angiogenesis, and metastasis. Objective:-This study investigates the potential effect of RECK gene promoter single nucleotide polymorphisms (SNPs) with HCC susceptibility on clinical and laboratory characteristics in Egyptian patients. Methods:-A number of 50 HCC cancer patients and 50 cancer-free controls were analyzed for RECK promoter rs10814325 genotyping using real-time PCR. Clinical staging and assay of AFP and liver function tests were performed for all participants. Results:-Having RECK promoter rs10814325 mutant genotypes TC/CC showed a 2.704 -fold [95% confidence interval (CI), 1.197 – 6.113] higher risk of developing HCC compared to TT wild type carriers. A significant association between clinical stage III/IV and RECK promoter rs10814325 mutant genotypes. However no significant association between laboratory parameters and RECK promoter genotypes. Conclusions:-RECK gene promoterpolymorphisms might be a risk factor increasing HCC susceptibility and clinical stage progression.