Vol. 4 (10) pp. 818-825 DOI: 10.21474/IJAR01/1863

RAPID DETECTION OF STAPHYLOCOCCUS AUREUS USING PAPER BASED MICROFLUIDIC DEVICES FOR RESOURCE LIMITED SETTINGS.

and
  • Ramnarain Ruia College, Microbiology Mumbai, Maharashtra, India.
83 Downloads 267 Views
Crossref

Download Full Paper (PDF)

Abstract

A micro?uidic paper-based analytical device (µPAD) has been developed for rapid detection of Staphylococcus aureus in resource limited settings. Staphylococcus aureus can be detected from food or clinical samples with enrichment in Sterile PYP broth for 1.5 h and used for detection of Alkaline Phosphatase activity using chromogenic substrate Blue Phos. The test is accompanied by Gram staining of the sample and coagulase test. Qualitative detection is based on visual detection while quantitative analysis is done using ImageJTM software. The test can detect up to 104 CFUmL-1 of Staphylococcus aureus within 3-4 h. This test is cost effective, does not need any trained personnel or sophisticated instruments for analysis. Analysis can be done on a smart phone. The test is ideal for presumptive diagnosis of Staphylococcus aureus for clinicians operating in remote locations and can be used as a screening test by food industries.

Keywords

Article Analytics

How to Cite This Article

Lokur Anushree and Phadke Ravindra. (2016); RAPID DETECTION OF STAPHYLOCOCCUS AUREUS USING PAPER BASED MICROFLUIDIC DEVICES FOR RESOURCE LIMITED SETTINGS., Int. J. of Adv. Res., 4 (10), 818-825, ISSN 2320-5407. DOI: https://doi.org/10.21474/IJAR01/1863

Corresponding Author

Ms. Anushree Lokur

Creative Commons Attribution 4.0 This work is licensed under a Creative Commons Attribution 4.0 International License.