EXPERIMENTAL DESIGN USE FOR ETHANOLIC TRANSESTERIFICATION OPTIMIZATION FOR BIODIESEL PRODUCTION CATALYZED BY FICUSTRICHOPODA LATEX LIPASE.

This work focused on optimization of biodiesel production from vegetal oil catalyzed by Ficustrichopoda latex using a response surface analysis. Ethanol substrate molar ratio, enzyme amount (latex), water activity, reaction duration, agitation speed and reaction temperature were examined. After use a box-Behnken design, the Plackett-Murman design were use as screening test to sort the factors while emphasizing most influential. The function of desirability shows that the value of the coefficient of maximum yield of EE estimated by the model (45,96%) corresponds to the values of the levels according to: aw = 0,63; latex rate = 24,85% ; reaction duration 7 days and molar ratio TG:Eth (1:5). It appears that the recycling of latexes of F. trichopoda as biocatalyst after two cycle catalytic of transesterification is not for the moment possible.