IN VIVO ASSESSMENT OF ANTI-ARTHRITIC ACTIVITY OF WITHANIA COAGULANS DUNAL FRUITS.

The aim of this study was to determine in-vivo anti-arthritic activity of methanolic and hydro alcoholic extract of Withania Coagulans Dunal fruits i.e. Withania Coagulans Methanolic Extract (WCME) and Withania Coagulans Hydro alcoholic Extract (WCHAE). Phytochemical analysis has indicated presence of alkaloids, flavonoids, glycosides, steroids, saponins, fixed oil, carbohydrates. Both the extracts have showed anti-arthritic activity by Complete Freund’s adjuvant induced arthritis (CFA) in rats method.Treatment with WCME-200mg/kg, WCME-500mg/kg, WCHAE-450 mg/kg caused significant (p<0.01) inhibition of the arthritis index score, paw edema volume, joint area and pain perception parameters like dorsal flexion and motility along with rise in stair climb score in comparison with disease control (CFA induced) groups on 28th day. Both the extracts showed the good anti-inflammatory activity but WCHAE showed the most significant anti-arthritic activity than WCME. The findings of the present study suggested that Withania Coagulans dunal fruits could be a potential natural source of ant-arthritis activity which is very important against treatment of rheumatoid arthritis disease.