Isolation and Characterization of a New Thermophilic, Carbazole Degrading Bacterium (Anoxybacillus rupiensis Strain Ir3 (JQ912241)

The main aim of this study was to isolate and identify thermophilic bacteria capable of utilizing aromatic hydrocarbon especially N-compounds that form part of petroleum components. A new aerobic Gram-variable long rod- shaped, thermophilic bacterium was isolated. The isolate cannot be distinguished by ordinary morphological, physiological and biochemical tests; therefore, it was subjected to molecular identification through PCR amplification of 16S rDNA by using 7 primers (fd1, fd2, fd3, fd4, rd1, rp1 and rp2) which represent primers for the PCR amplification of eubacterial 16S rDNA, and followed by sequencing. The nucleotide sequence data was compared with 16S rDNA sequences of other culture on BLAST of the National Center of Biotechnology Information database (NCBI database). According to the results of molecular identification, the isolate characterized as Anoxybacillus rupiensis strain (Ir3), and deposited in the National Genebank database under the accession number, (JQ912241). Optimum conditions for growth of A. rupiensis strain Ir3 (JQ912241) were determined. Results showed that growing in LB medium (pH 7) containing 0.5 to 1% of NaCl, and incubated with shaking (150 rpm) at 55 -65?C for 24h. It was also found that this bacterium was able to withstand 80?C for 90 min. The optimum conditions for growth of A. rupiensis strain Ir3 (JQ912241) in minimal medium (CDM) were adjusted, the pH to 7, incubated at 55-65?C and the bacterial growth was increased with carbazole concentration increased. This means that bacterial growth with CAR as an N-source was concentration-dependent.