Antibacterial Effect of Silver & Gold Nanoparticles and Diode Laser against Lactobacillus acidophilus bacteria

The presence of necrotic tissue and bacteria may cause the persistence of infection in root canals. The aim of this study is to assess the antibacterial effect of diode laser irradiation, silver nanoparticles, gold nanoparticles and combination between them on extracted single rooted teeth. A reference strain of anaerobic L.acidophilic bacteria was irradiated at different time (30 seconds, 1 minute, 2 minutes, and 3 minutes). After the tests were performed, the number of colony-forming units per milliliter (CFU/ml) was counted and the results were statistically analyzed. According to the data evaluated, laser and silver groups showed the lowest mean bacterial count, while no statistical difference between silver groups, gold groups ,and laser + gold groups.