20Jan 2017

AMELIORATIVE EFFECT OF THE AURELIA AURITA VENOUM ON THE MURINE EHRLISH ASCITES CARCINOMA-INDUCED HEPATOTOXICITY AND NEPHROTOXICITY.

  • Zoology Department, Faculty of Science, Port Said University, Port Said, Egypt.
  • Biology Department,Faculty of Science, Al-Jouf University., Sakaka, Saudi Arabia.
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The ability of Jellyfish, Aurelia aurita (A.A) crude venom to modify hepatic and renal dysfunction associated with Ehrlish ascites carcinoma ( EAC) stress in experimental mice was assessed. Intraperitoneal injection of A.A. crude venom into EAC-bearing mice at different doses (50, 125, 250, 500,750 mg/kg B.W), daily, for 7 days was compared with the standard drug, cisplatin, (2mg/kg) . Loading the mice with EAC significantly reduced the red blood cells count s and subsequently reduced the haemoglobin (Hb). Administration of A.A venom significantly restored red blood count and Hb. EAC load had increased the activities of serum AST and ALT but decreased the concentration of albumin and total protein. A.A. crude venom as well as cisplatin improved the hepatic injury by declining the ALT activity and increasing albumin values but no significant changes in the total protein were observed. Moreover, levels of the renal biomarkers, creatinine and urea were highly elevated in EAC-bearing mice. Interestingly unlike cisplatine, levels of those renal biomarkers, creatinine and urea, were significantly decreased by A.A. crude venom administration . Furthermore, histopathological examinations revealed hepatic and renal tissues improvement in EAC-loaded mice after venom administration compared with control group. Taken together, results from thisstudy suggested that Aurelia aurita crude venom can potentially reduce the hepatic and renal damage induced by EAC load in experimental animals.

  1. Abd El-Aziz, M.E.Hefni and Amira M. Shalaby (2014): Inhibitory effects of Rosemary (Rosmarinus officinalis L.) on Ehrlich ascites carcinoma in mice. Int.J.Curr.Res.Aca.Rev.2014; 2(9):330-357.
  2. Abu-Amra S. (2000): Physiological studies on diabetic male albino mice treated with bradykinin potentiating factor (BPF 7) separated from jellyfish and cobra snake venoms. J. Egypt Ger. Soc. Zool., 31(A): 211-233.
  3. Abu-Amra S., and Abd El-Rehim S.A (1992): Effect of factors isolated from venom of Egyptian snakes on plasma cholesterol, creatine kinase and alkaline phosphatase. J. Ger. Soc. Zool., 9(A):75-89
  4. Abu-Amra E., Abd-EL-Rehim S.A., Lashein F.M and Seleem A.A (2015): Influence of Bradykinin potentiating factor on protecting liver and kidney against the toxicity of indomethacin in male mice. Issue 5 1.625.
  5. Armstrong D.K (2006): I.P cisplatin and paclitaxel in ovarian cancer.N Engl J Med 354, 34-43.
  6. Badre, S. (2014): Bioactive toxins from stinging jellyfish, Toxicon.09.010.
  7. Bawaskar, H. (2012): Scorpion Sting: Update. J Assoc Physicians India 60,46-53.
  8. Bruschetta G., Impellizzeri D., Morabito R, Marino A., Ahmad A., Span? N., La Spada G., Cuzzocrea S. and Esposito E. (2014): Pelagia noctiluca (Scyphozoa) Crude Venom Injection Elicits Oxidative Stress and Inflammatory Response in Rats. Mar Drugs, 12, 2182-2204.
  9. Chakraborty T, Chatterjee A, Saralaya M.G and Chatterjee M., (2006): Chemopreventive effect of vanadium in a rodent model of chemical hepatocarcinogenesis: reflectionsin oxidative DNA damage, energy- dispersive X-ray fluorescence profile and metallothionein expression. J. Biol. Inorg. Chem. 11(7): 855-866.
  10. DeWys W.D (1982): Pathophysiology of cancer cachexia:? current understanding and areas for future research. Cancer Res. 42(2):721s.
  11. El-Dayem S.M, Fouda F.M, Ali E.H and Motelp B.A (2013): The antitumor effects of tetrodotoxin and/or doxorubicin on Ehrlich ascites carcinoma bearing female mice. Toxicol Indus health, 29, 404417.
  12. El-Naggar S.A (2011): Lack of the beneficial effects of Mirazied (commiphoramolma) when administered with chemotherapeutic agents on Ehrlich a scetic carcinoma bearing mice. Adv Biol Ress,193-199
  13. Einhorn E.H (1997): Testicular cancer: an oncological success ? Clin Cancer Res 3, 2630-2632.
  14. Enjalbert A, Arancibia S, Ruberg M, Priam M, Bluet-Pajot M.T, Rotsztein W.H and Kordon C (1980): Stimulation of in vitro prolactin release by vasoactive intestinal peptide. Neuroendocrinology, 31:200-204.
  15. Etgen A.M and Browning E.T (1983): Activators of cyclic adenosine 3′:5- monophosphate accumulation in rat hypocampal slices: Action of vasoactive intestinal peptide (VIP). ? Neurosci., 3(12): 2487-2493.
  16. Fenninger L.D and Mider G.B (1954): Advances in cancer research. Grenstein JP, Haddow A, editors. v2. New York: Academic Press; 1954. p244.
  17. Garrison R.K, Galloway R.H and Heuser L.S (1987): Mechanism of malignant ascites production. J Surg Res 42, 126-132.
  18. Geraci J.P, Jackson K.L, Mariano M.S and Michieli B.M (1990): Kidney and lung injury in irradiated rats protected from acute death by partial ?body shielding. Rad. Res., 112(1), 95.
  19. Gupta M, Mazumder U.K, Kumar R.S and Kumar T.S (2004): Antitumor activity and antioxident role of Bauhinia racemosa against Ehrlich ascites carcinoma in Swiss albino mice. Acta Pharmacol Sin 25:1070?1076.
  20. Helm C.W and States J.C (2009): Enhancing the efficacy of cisplatin in ovarian cancer treatment could arsenic have a role. J Ovarian Res 2, 2.
  21. Jakowska S.R.F, Nigrelli R.F, Murray P.M and Veltri A (1958): Hemopoietic effects of Holothurin, a steroid saponin from the sea cucumber, Actinopyga agassizi, in Rana pipiens. Anat. Res., 132: 456-466.
  22. Kim SK and Wijesekara I (2010): Development and biological activities of marine derived bioactive peptides: a review. J. Functional Foods., 2(1): 1-9.
  23. Konca C, Tekin M, Colak P, Uckardes F, Turgut M (2014): An overview of platelet indices for evaluating platelet function in children with scorpion envenomation. Exp Clin Sci 67, 801-808.
  24. Konstantakou E.G (2009). Human bladder cancer cells undergo cisplatin-induced apoptosis that is associated with p53-dependent and p53-independent responses. Int J Oncol 35, 401-416.
  25. Lee H, Jung E, Kang C, Yoon W.D, Kim J.S and Kim E (2011): Scyphozoan jellyfish venom metalloproteinases and their role in the cytotoxicity. Toxicon 58,
  26. Lenth R.V (1989): Quick and Easy Analysis of Unreplicated Factorials. Technometrics, Vol31, p.469.
  27. Liang X., Sihua Liu, Qian He, Qianqian Wang, Xuting Ye, Guoyan Liu, Fei Nie, Jie Zhao, and Liming Zhang (2011): The Acute Toxicity and Hematological Characterization of the effects of Tentacle-Only Extract from the Jellyfish Cyanea capillata. Mar. Drugs, 9, 526-534
  28. Meenakshi S., Paripoorana S., Gomathy S. and Chamundeswari (2013): In vitro and in vivo antitumor and immunomodulatory studies of Microcosmus exasperatus against DLA bearing mice. European Journal of Applied Engineering and Scientific Research, 2 (3):18-25.
  29. Morabito R, La Spada G, Crupi R, Esposito E and Marino A (2015): Crude Venom from Nematocysts of the Jellyfish Pelagia noctiluca as a Tool to Study Cell Physiology. Cent. Nerv. Syst. Agents. Med. Chem., 15, 6873.
  30. Nagarjuna Reddy, Nagarathna P.K.M and M Divya (2013): Evaluation of anti-cancer activity of Ruellia tuberosa on EAC induced mammary tumor International Journal of Pharmacology and Toxicology, 1 (2) 36-42
  31. Natesan S, Badami S, Dongre S.H and Godavarthi A (2007): Antitumor activity and antioxidant status of the methanol extract of Careya arborea bark against Dalton?s lymphoma ascites induced ascitic and solid tumor in mice. J Pharmacol Sci 103:12?23.
  32. Nevalainen T.J, Peuravuori H.J, Quinn R.J, Llewellyn L.E, Benzie J.A.H, Fenner P.J and Winkel K.D (2004): Phospholipase A2 in Cnidaria. Comp. Biochem. Physiol. B Biochem.? Biol. 139, 731e735
  33. Pabla N. and Dong Z (2008): Cisplatin nephrotoxicity: mechanisms and renoprotective strategies. Kidney Int 73, 994-1007.
  34. Price V.E and R.E Greenfeild (1954): Anemia in cancer. In: Advanced in Cancer Research. Greenstein J.P. and A. Haddow (Eds), New York: Academic Press, pp: 244.
  35. Radwan F,? Burnett? J.W,? Bloom? D.A,? Coliano T, Eldefrawi M.E, Erderly H, Aurelian L, Torres M and Heimer-de la Cotera E.P (2001):A comparison of the toxinological characteristics of two Cassiopea and Aurelia species. Toxicon 39, 245-257.
  36. Rastogi A, Biswas S, Sarkar A and Chakrabarty D (2012): Anticoagulant activity of Moon jellyfish (Aurelia aurita) tentacle extract. Toxicon 60, 719-723.
  37. Senthilkumar N, Badami S, Dongre S.H and Bhojraj S (2008): Antioxidant and hepatoprotective activity of the methanol extract of Careya arborea bark in Ehrlich ascites carcinoma bearing mice. Ethnopharmacol. 116(1), 1-6.
  38. Sinclair A.J, A.H Barnett and J Luine (1990): Free radical and autooxidant system in health and disease. Br. Hosp. Med., 43: 334-344.
  39. Sundaram M, Patra? S and Maniarasu? G (2012): Antitumor? activity? of ethanol? extract? of? Gracilaria? edulis? (Gmelin)? Silva? on? Ehrlich ascites carcinoma- bearing? Journal of Chinese Intrgrative Medicine, 10 (4): 430-435
  40. Ponce D, Lopez-Vera E, Aguilar M.B and Sanchez-Rodr?guez J (2013): Pre-liminary results of the in Vivo and in Vitro characterization of a tentacle venom fraction from the jellyfish Aurelia aurita. Toxins 5, 2420-2433
  41. Segura-Puertas L, Avila-Soria G, Sanchez-Rodr?guez J, Ramos Aguilar M.E and Burnett J.W (2002): Some toxinological aspects of Aurelia aurita (Linne) from the Mexican Caribbean. ? Venom. Anim.Toxins8, 269-282.
  42. Torres M and Heimer E (2001): A comparison of the toxinological characteristics of two Cassiopea and Aurelia Toxicon 39, 245-257.
  43. Zaki M.A (2005): Effects of the crude toxin of sea Cocumbers Holothuria atra on some hematological and biochemical parameters in rats. Egyptian Journal of Natural Toxins, Vol. 2, 71-86.

[Sherifa H. Ahmed, Doaa M. Eissa, Mohamed K. Hassan and Osama A. Abaas. (2017); AMELIORATIVE EFFECT OF THE AURELIA AURITA VENOUM ON THE MURINE EHRLISH ASCITES CARCINOMA-INDUCED HEPATOTOXICITY AND NEPHROTOXICITY. Int. J. of Adv. Res. 5 (Jan). 1167-1178] (ISSN 2320-5407). www.journalijar.com

Doaa Mohammed Eissa
Zoology Department, Faculty of Science, Port Said University

DOI:

Article DOI: 10.21474/IJAR01/2867      
DOI URL: https://dx.doi.org/10.21474/IJAR01/2867

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