30Jan 2017

COMPARATIVE STUDIES OF GENETIC VARIABILITY IN SOYBEAN (GLYCINE MAX. (L.) MERRILL) DERIVED GENOTYPES USING MOLECULAR MARKER.

  • Seed Testing Division, Department of Quality Assurance, Ajeet Seeds Pvt Ltd, Chitegaon, Aurangabad.
  • Department of Biotechnology, Government Institute of Science, Aurangabad.
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The vast Mutagenic effect showed by application of mutagens (Sodium Azide -3mM, 5mM, 7mM; Ethyl Methane Sulphonate -5mM, 10mM, 15mM; And Gamma Irradiation -100kr, 200kr, 300kr).That clearly showed in field as well as laboratory testing; by using molecular markers, PCR (Polymerase chain reaction) ISSR( Inter Simple Sequence Repeat). Mutagenic variation founded in all the mutagens, but selected Genotypes were obtained from 100kr and 10mM Mutagenic lines. We demonstrate these genotypes for phenotypic screening and associated maturity, morphology, pigmentation traits. ISSR analysis done by two Primers and it was detected polymorphisms. For analysis, two primers were designed from consensus sequence regions on protein homolog genes, and used to amplify the genomic region. PCR samples were sequenced. The total number of polymorphic ISSR bands showed specific range and scored on all genotypes and their homology was measured.

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[Sanjay N. Pathare and Rajendra A. Satpute. (2017); COMPARATIVE STUDIES OF GENETIC VARIABILITY IN SOYBEAN (GLYCINE MAX. (L.) MERRILL) DERIVED GENOTYPES USING MOLECULAR MARKER. Int. J. of Adv. Res. 5 (Jan). 1984-1989] (ISSN 2320-5407). www.journalijar.com

Sanjay Pathare
Department of Biotechnology, Government Institute of Science, Aurangabad.

DOI:

Article DOI: 10.21474/IJAR01/2969      
DOI URL: https://dx.doi.org/10.21474/IJAR01/2969

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