30Jan 2017

EFFLUX AS AN ARISING CAUSE OF DRUG RESISTANCE IN E. COLI.

  • Department of Microbiology Shoolini University of Biotechnology and Management Sciences, Solan, Himachal Pradesh, India.
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Multidrug resistant strains of bacteria which are characterized as serious public health problem may arise due to over expression of efflux pumps. Efflux pumps in bacteria can be detected by using specialized instruments. In the present study simple instrument free agar cartwheel method was used to detect efflux pumps in E.coli with some modification. Four isolates of E.coli were collected and processed. Biochemical analysis of all the strains was done using standard protocol. Further cartwheel assay was performed. MIC method was used to confirm the observations of agar cartwheel assay. In Cartwheel assay, a total of 4 strains of E.coli were analyzed for the presence of efflux pumps, active efflux pump was detected in 1 isolate while it was not observed in 3 strains. Bacterial isolate which contains efflux pump also showed high MIC value for the antibiotics used. But in the presence of an EPI, a considerable decrease in the MIC of antibiotics was observed among isolate containing efflux pump, while there was no decrease in MIC of antibiotics for strains without efflux pumps. Present study revealed that cartwheel assay to detect efflux pumps is a reliable, fast and sensitive technique, which may reduce time and efforts to detect efflux pumps.

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[Rakesh kumar Savita Jandaik and Pooja Patial. (2017); EFFLUX AS AN ARISING CAUSE OF DRUG RESISTANCE IN E. COLI. Int. J. of Adv. Res. 5 (Jan). 1999-2007] (ISSN 2320-5407). www.journalijar.com

RAKESH KUMAR
Department of Microbiology Shoolini University of Biotechnology and Management Sciences, Solan, Himachal Pradesh, India.

DOI:

Article DOI: 10.21474/IJAR01/2971      
DOI URL: https://dx.doi.org/10.21474/IJAR01/2971

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